Time resolved fluorescent imaging system

Details Time resolved fluorescent imaging system Time resolved fluorescent imaging system

2008-01-23

2011-11-22

Hannaher, Constantine (Department: 2884)

Radiant energy

Luminophor irradiation

Methods

Reexamination Certificate

active

08063386

ABSTRACT:
A system and method that allows for time-resolved fluorescent imaging of fluorescent samples. The user is able to receive temporally filtered pictures of the sample with a reduced amount of the scattered excitation light and the short lived background fluorescence. The system allows for adjustment of fluorescent gating time and delay time.

REFERENCES:
patent: 5523573 (1996-06-01), Hanninen et al.
patent: 6504167 (2003-01-01), Ikami
patent: 7794661 (2010-09-01), Ikami
patent: 2002/0001075 (2002-01-01), Tsien et al.
patent: 2003/0160151 (2003-08-01), Zarate et al.
patent: 2006/0017001 (2006-01-01), Donders et al.
patent: WO 00/08443 (2000-02-01), None
Austin, R. H., et al., “Rotational diffusion of cell surface components by time-resolved phosphorescence anisotropy,” Proc Natl Acad Sci. 76, 5650-5654 (1979).
Bigas, M., et al., “Review of CMOS image sensors,” Microelectronics J 37, 433-451 (2006).
Clegg, R. M., “Fluorescence lifetime-resolved imaging: Measuring lifetimes in an image,” Methods in Enzymology, 360, 509-542 (2003).
El Gama, A., et al., “CMOS image sensors,” IEEE Circuits & Devices 21, 6-20 (2005).
Elson, D., et al., “Time-domain fluorescence lifetime imaging applied to biological tissue,” Photochem & Photobio Sci, 3 (8) 795-801 (2004).
Hemmila, I., et al., “Time-resolution in fluorometry technologies, labels and applications in bioanalytical assays,” Crit Rev Clin Lab Sci 38, 441-519 (2001).
Hemmila, I., et al., “Progress in lanthanides as luminescent probes,” J Fluores 15, 529-542 (2005).
Krishnan, R. V., et al., “Development of multiphoton fluorescence lifetime imaging microscopy (FLIM) system using a streak camera,” Rev. Sci. Instrum., 74, 2714-2721 (2003).
Lakowicz, J. R., et al., “Microsecond dynamics of biological macromolecules,” Methods Enzym. 323, 473-509 (2000).
Owicki, J. C., “Fluorescence polarization and anisotropy in high throughput screening: perspective and primer,” J Biomol Scr 5, 297-306 (2000).
Ramm, P., “Image-based screening: a technology in transition,” Curr. Opin. Biotech. 16, 41-48 (2005).
Schauerte, J. A., et al., “Time-resolved room temperature tryptophan phosphorescence in proteins,” Methods Enzym. , 278, 49-71 (1997).
Seveus, L., et al., “Time-resolved fluorescence imaging of europium chelate label in immunohistochemistry and in situ hybridization,” Cytometry, 13, 329-338 (1992).
Soini, A. E., et al., “A new technique for multiparametric imaging microscopy: Use of long decay time photoluminescent labels enables multiple color immunocytochemistry with low channel to-channel crosstalk,” Microsc Res Technol, 62, 396-407 (2003).
Subramaniam, V., et al., “Time-resolved tryptophan phosphorescence spectroscopy: A sensitive probe of protein folding and structure,” IEEE J of Selected Topics in Quantum Electronics 2, 1107-1114 (1996).
Suhling, K., et al., “Time-resolved fluorescence microscopy,” Photochem & Photobio Sci, 4 (1) 13-22 (2005).
Vanderkooi, J. M., et al., “On the prevalence of room temperature protein phosphorescence,” Science 236, 568-569 (1987).
Vanderkooi, J. M., Tryptophan phosphorescence from proteins at room temperature, In “Topics in Fluorescence Spectroscopy,” vol. 3 Biochemical Applications, Lakowicz JR Edited, Plenum Press, NY, 1992, pp. 113-136.
Van Munster, E. B., et al., “Fluorescence lifetime imaging microscopy (FLIM),” Adv in Biochem Eng / Biotech, 95, 143-175 (2005).
Zhou, X., et al., “High content cellular imaging for drug development,” IEEE Sig. Proc. Mag. 23, 170-174 (2006).

Affiliated with

Also associated with

Rating

Time resolved fluorescent imaging system does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Time resolved fluorescent imaging system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Time resolved fluorescent imaging system will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4266142