Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation
Patent
1997-07-29
1999-06-01
Font, Frank G.
Optics: measuring and testing
By dispersed light spectroscopy
With sample excitation
2504611, 2504612, 436172, 356 73, G01J 330, G01N 2164, G01N 2176
Patent
active
059092785
ABSTRACT:
Time-resolved fluorescence decay measurements for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated cw laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes.
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Deka Chiranjit
Steinkamp John A.
Font Frank G.
Freund Samuel M.
Smith Zandra V.
The Regents of the University of California
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