Time-of-flight mass spectrometry analysis of biomolecules

Radiant energy – Ionic separation or analysis – Ion beam pulsing means with detector synchronizing means

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250282, 250423P, B01D 5944, H01J 4900

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active

056251840

ABSTRACT:
A time-of-flight mass spectrometer for measuring the mass-to-charge ratio of a sample molecule is described. The spectrometer provides independent control of the electric field experienced by the sample before and during ion extraction. Methods of mass spectrometry utilizing the principles of the invention reduce matrix background, induce fast fragmentation, and control the transfer of energy prior to ion extraction.

REFERENCES:
patent: 4730111 (1988-03-01), Vestal et al.
patent: 4731533 (1988-03-01), Vestal
patent: 4766312 (1988-08-01), Fergusson et al.
patent: 4814612 (1989-03-01), Vestal et al.
patent: 4861989 (1989-08-01), Vestal et al.
patent: 4883958 (1989-11-01), Vestal
patent: 4902891 (1990-02-01), Vestal
patent: 4958529 (1990-09-01), Vestal
patent: 4960992 (1990-10-01), Vestal et al.
patent: 4999493 (1991-03-01), Allen et al.
patent: 5015845 (1991-05-01), Allen et al.
patent: 5032722 (1991-07-01), Boesl et al.
patent: 5045694 (1991-09-01), Beavis et al.
patent: 5118937 (1992-06-01), Hillenkamp et al.
patent: 5135870 (1992-08-01), Williams et al.
patent: 5144127 (1992-09-01), Williams et al.
patent: 5160840 (1992-11-01), Vestal
patent: 5180914 (1993-01-01), Davis et al.
patent: 5210412 (1993-05-01), Levis et al.
patent: 5245185 (1993-09-01), Busch et al.
patent: 5288644 (1994-02-01), Beavis et al.
patent: 5376788 (1994-12-01), Standing et al.
patent: 5396065 (1995-03-01), Myerholtz et al.
patent: 5464985 (1995-11-01), Cornish et al.
J.J. Lennon et al., "Pulsed Ion Extraction Combined with High Accelerating Potentials for Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry", Proceedings The 42nd ASMS Conference on Mass Spectrometry and Allied Topics, May 29-Jun. 3, 1994, Chicago, Illinois, p. 501 (Abstract).
R.T. Pogue et al., "Appliations of X-Ray for Ionization and Fragmentation in Time-of-Flight Mass Spectrometry", Proceedings The 42nd ASMS Conference on Mass Spectrometry and Allied Topics, May 29-Jun. 3, 1994, Chicago, Illinois, p. 748 (Abstract).
P. Juhasz et al., "Applications of High Resolution MALDI TOF to Oligonucleotide Problems", Proceedings The 43rd ASMS Conference on Mass Spectrometry and Allied Topics, May 21-26, 1995, Atlanta, Georgia, p. 364 (Abstract).
D. Reiber et al., "Fast Metastable Ion Fragmentation in MALDI", Proceedings The 43rd ASMA Conference on Mass Spectrometry and Allied Topics, May 21-26, 1995, Atlanta, Georgia, p. 683 (Abstract).
W. Ens et al., "Delayed Extraction in Matrix-Assisted Laser Desorption" Proceedings The 43rd ASMS Conference on Mass Spectrometry and Allied Topics, May 21-26, 1995, Atlanta, Georgia, p. 684 (Abstract).
M. Vestal et al., "Probing the Outer Limits of Performance for MALDI-TOF MF and MS-Ms", Proceedings The 43rd ASMS Conference on Mass Spectrometry and Allied Topics, May 21-26, 1995, Atlanta, Georgia, p. 1209 (Abstract).
D. Reiber et al., "Hydroxy Benzophenones: A New Class of MALDI Matrices for Proteins/Peptides", Proceedings The 43rd ASMS Conference on Mass Spectrometry and Allied Topics, May 21-26, 1995, Atlanta, Georgia, p. 1249 (Abstract).
R.J. Cotter, "Progress and Prospects for TOF Mass Spectrometry" A Workshop Sponsored by the Time-of-Flight Interest Group, May 22, 1995, Proceedings The 43rd ASMS Conference on Mass Spectrometry and Allied Topics, May 21-26, 1995, Atlanta, Georgia, p. 1364 (Abstract).
K. Breuker et al., "Pulsed Ion Extraction to Improve the Mass Resolution in a Low-Acceleration-Energy Linear-TOF MALDI Instrument" Abstract from 13th Int'l Mass Spectrometry Conference, Aug. 29-Sep. 2, 1994, Budapest Hungray, Th B24.
R.S. Brown et al., "Pulsed Ion Extraction with High Source Accelerating fields for MALD Time-of-Flight Mass Spectrometry" Desorption of Mass Spectrometry of Large Organic Ions by Particle and Photon Induced Desorption Sunriver Lodge and Resort, OR (Mar. 27-31, 1994).
C.H.W. Chen et al., "Laser Desorption Mass Spectrometry for Fast DNA Sequencing" Contractor-Grantee Workshop IV, Nov. 13-17, 1994, Santa Fe, NM (published Oct. 1994).
S.M. Colby, "Improving the Resolution of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry by Exploiting the Correlation Between Ion Position and Velocity" Rapid Comm. in Mass Spectrom. 8:865-868 (1994).
R.J. Cotter et al., "Structural Determinatin of Lipid A from Gram Negative Bacteria Using Laser Desorption Mass Spectrometry" Biomed. and Environ. Mass Specgtrometry 14:591-598 (1987).
R. J. Cotter, "Time-of-Flight Mass Spectrometry: An Increasing Role in the Life Sciences" Biomed. and Environ. Mass Spectrometry 18:513-532 (1989).
R.J. Cotter, "Time-of-Flight Mass Spectrometry for the Structural Analysis of Biological Molecules" Anal. Chem. 64(21):1027-1039 (1992).
R.J. Cotter, Third Int'l Symposium on Mass Spectrometry in the Life Sciences, San Francisco, CA, Sep. 1994.
R.J. Cotter, letter to Noubar B. Afeyan, Ph.D. of PerSeptive Biosystems, Inc., dated May 31, 1995.
G.J. Currie et al., "Analysis of Oligodeoxynucleotides by Negative-Ion Matrix-Assisted Laser Desorption Mass Spectrometry" J. Am. Soc. Mass Spectrom. 4:955-963 (1993).
M.C. Fitzgerald et al., "Basic matrices for the Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Proteins and Oligonucleotides" Anal. Chem. 65:3204-3211 (1993).
M.C. Fitzgerald et al., "The Analysis of Mock DNA Sequencing Reactions Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry" Rapid Comm. in Mass Spectrom. 7:895-897 (1993).
M.C. Fitzgerald et al., "The Development of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for DNA Sequencing" Abstracts and Notes ASMS, Spring 1994.
D.N. Heller et al., "Mass Spectral Analysis of Complex Lipids Desorbed Directly from Lyophilized Membranes and Cells" Biochem. and Biophys. Res. Comm. 142(1):194-194-199 (1987).
T. Huth-Fehre et al., "Matrix-Assisted Laser Desorption Mass Spectrometry of Oligodeoxythymidylic Acids" Rapid Comm. in Mass Spectrom. 6:209-213(1992).
K.B. Jacobson et al., "An Approach to the Use of Stable Isotopes for DNA Sequencing" Genomics 9:51-59 (1991).
K.B. Jacobson et al., "Applications of Mass Spectrometry to DNA Sequencing" GATA 8(8):223-229 (1991).
T. Keough et al., "Antisense DNA Oligonucleotide II: the Use of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for the Sequence Verification of Methylphosphonate Oligodeoxyribonucleotides" Rapid Comm. in Mass Spectrom. 7:195-200 (1993).
G.R. Kinsel et al., "Resolution Enhancement in Linear Time-of-Flight Mass Spectrometry by Post-Source Pulse Focusing" Intl. J. of Mass Spectrom. and Ion Processes 104:35-44 (1991).
S.A. McLuckey et al., "Tandem Mass Spectrometry of Small, Multiply Charged Oligonucleotides" J. Am. Soc. Mass Spectrom. 3:60-70 (1992).
O.A. Mirgorodskaya et al., "Electrospray Ionization Time-of-Flight Mass Spectrometry in Protein Chemistry" Anal. Chem. 66(1):99-107 (1994).
E. Nordhoff et al., "Role of Sample Preparation and Purification for UV-and IR MALDI of Oligonucleotides" Institute for Medical Physics and Biophysics, Univ. of Munster, Germany.
Pan et al., "Measurement of Initial Translational Energies of Pepide Ions in Laser Desorption/Ionization Mass Spectrometry" Org. Mass. Spectrom. 27:3(1992).
U. Pieles et al., "Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry: A Powerful Tool for the Mass and Sequence Analysis of Natural and Modified Oligonucleotides" Nucleic Acids Research 21(14):3191-3196 (1993).
P.T.A. Reilly et al., "Laser Desorptin/Gas-Phase Ionization of the Amino Acid Tryptophan" Rapid Comm. in Mass Spectrom. 8:731-734 (1994).
K. Schneider et al., "Matrix-Assisted Laser Desorption Mass Spectrometry of Homopolymer Oligodeoxyribonucleotied. Influence of Base Composition on the Mass Spectrometric Reponse" Org. Mass Spectrom. 28:1353-1361 (1993).
J.M. Schuette et al., "Sequence Analysis of Phosphorothioate Oligonucleotides via Matrix-Assisted Laser Desorption Ionization Time-of-Fight Mass Spectrometry".
B. Spengler et al., "Ultraviolet Laser Desorption/Ionization Mass Spectrometry of Proteins Above 100,000 Daltons by Pulsed Ion Extraction Time-of-Flight Analysis" Anal.

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