Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1975-03-10
1977-04-19
Padgett, Benjamin R.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
23230B, 424 15, G01N 3300, A61K 4300
Patent
active
040188833
ABSTRACT:
It has been discovered polyethylene glycol can be added as a separator to the other reagents of a T.sub.4 radioimmunoassay of unextracted serum prior to introduction of the serum, without impairing the sensitivity of the measurement. A single, highly stable composite reagent is prepared to measure the concentration of T.sub.4 in a measured quantity of human serum; the composite reagent comprises, in freeze dried form, a blocking agent in sufficient quantity to displace essentially all the T.sub.4 in the measured quantity of serum bound to TBG, an antibody in sufficient quantity to bind a significant quantity of the T.sub.4 in the measured quantity of serum, PEG in sufficient quantity to precipitate essentially all the antibody, and a buffering agent in sufficient quantity to inhibit binding the T.sub.4 in the measured quantity of serum to TBP. To run a radioimmunoassay, the human serum being assayed and quantities of standard serums containing known concentrations of T.sub.4 are added to a mixture of the composite reagent reconstituted with water and radioactive T.sub.4 in sufficient quantity to give a measurable counting rate. After an incubation period, the antibody precipitated by the PEG, including the antibody bound T.sub.4, is removed by centrifuging and aspirating the supernatants. The radio activity of the precipitates is then counted to correlate the T.sub.4 in the serum being assayed with the standard serums.
REFERENCES:
patent: 3911096 (1975-10-01), Chopra
Chopra, Journal of Clinical Endocrinology and Metabolism, vol. 34, 1972, pp. 938-947.
Radioimmunoassay Manual, Nichols Institute for Endocrinology, San Pedro, Calif., Jan. 1974, pp. 15-17.
Desbuquois et al, Journal of Clinical Endocrinology and Metabolism, vol. 33, 1971, pp. 732-738.
Werner et al, Journal of Clinical Endocrinology and Metabolism, vol. 38, 1974, pp. 493-495.
Nichols Institute for Endocrinology
Nucker Christine M.
Padgett Benjamin R.
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