Thyroid polarization fluoroimmunoassay

Details Thyroid polarization fluoroimmunoassay Thyroid polarization fluoroimmunoassay

1980-09-15

1982-08-31

Marcus, Michael S.

Chemistry: physical processes

Physical processes

Crystallization

23923, 424 15, 435 7, 435 8, G01N 3352, G01N 3354, G01N 3378

Patent

active

043470580

ABSTRACT:
A polarization fluoroimmunoassay for T.sub.3 or T.sub.4 thyroid hormones where a serum sample is assayed by contacting the sample with a base to denature the thyroxine binding globulins and thereby form a solution, incubating the solution, contacting the solution with a separating agent and separating bound thyroid hormone from the serum, contacting the separating agent with bound thyroid hormone with an antibody against the thyroid hormone, incubating the separating agent-antibody combination, separating free antibody and thyroid hormone bound antibody from the separating agent, contacting the free antibody with fluorescent labelled thyroid hormone and measuring the fluorescence polarization as an indication of the thyroid hormone in the sample.

REFERENCES:
patent: 3776698 (1973-12-01), Eisentraut
patent: 3911096 (1975-10-01), Chopra
patent: 3941564 (1976-03-01), Fader et al.
patent: 4272505 (1981-06-01), Smith
Murphy et al., J. Clin. Endocrinol., 24:187, (1964).
Braverman et al., J. Clin. Endocrinol., 32:497, (1971).
Alexander et al., Clin. Chem., 20:553, (1974).
Seth et al., Clin. Chem., 21:1406, (1975).

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