Thyroglobulin quantitation by mass spectrometry

Chemistry: analytical and immunological testing – Peptide – protein or amino acid

Reexamination Certificate

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C435S004000, C435S007100, C435S007800, C436S171000, C436S173000

Reexamination Certificate

active

08030084

ABSTRACT:
Provided are methods for determining the amount of thyroglobulin in a sample using various purification steps followed by mass spectrometry. The methods generally involve purifying thyroglobulin in a test sample, digesting thyroglobulin to form peptide T129, purifying peptide T129, ionizing peptide T129, detecting the amount of peptide T129 ion generated, and relating the amount of peptide T129 ion to the amount of thyroglobulin originally present in the sample.

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