Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1978-10-10
1980-08-05
Naff, David M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
23230B, 260112R, 424 12, 435 13, C07G 700, C07G 702, G01N 3114, G01N 3316
Patent
active
042162912
ABSTRACT:
A thrombosis test is based on the presence of specific, newly-found, enzyme-inhibitor complexes (such as plasmin-antiplasmin, plasmin-.alpha..sub.2 -macroglobulin, thrombin-antithrombin-III) in blood samples having an activated blood coagulation and/or fibrinolytic system. The test is immunochemical and uses a purified antiserum which has been generated against the enzyme-inhibitor complex. The antiserum is purified by incubating the antiserum with plasminogen and fresh blood plasma and isolating a gamma globulin fraction containing antibodies specific to the enzyme-inhibitor complex. A reagent for the test is prepared by contacting a blood cell suspension or a latex of particles of synthetic resin with the purified antiserum to obtain a suspension or latex in which antibodies from the antiserum are present on the surface of the cells or particles.
REFERENCES:
patent: 3912805 (1975-10-01), Cayzer et al.
Collen et al., A Tanned Red Cell Hemagglutination Inhibition Immunoassay (TRCHII) for the Quantitative Estimation of Thrombin-Antithrombin III and Plasmin-Alpha-Antiplasmin Complexes in Human Plasma, Thrombosis Research, vol. 7 1975, pp. 235-238.
Collen, D., Emergence in Plasma During Activation of the Coagulation or Fibrinolytic Systems of Neoantigens, Associated with the Complexes of Thrombin or Plasmin with their Inhibitors, Thrombosis Research, vol. 5, 1974, pp. 777-779.
Collen et al., Immunochemical Distinction Between Antiplasmin and Alpha.sub.1 -Antitrypsin, Thrombosis Research, vol. 7 1975, pp. 245-249.
Merskey et al., A Rapid, Simple, Sensitive Method for Measuring Fibrinolytic Split Products in Human Serum, Proc. Soc. Exp. Biol. Med., vol. 131 1969, pp. 871-875.
Eslinger Lewis H.
Leuven Research & Development v.z.w.
Naff David M.
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