Thrombolytic agent and process of preparation thereof

Details Thrombolytic agent and process of preparation thereof Thrombolytic agent and process of preparation thereof

1993-09-09

1995-07-18

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435 711, 435 713, 435212, 435217, 435221, 435226, C12P 2104, C12N 948, C12N 954

Patent

active

054340594

ABSTRACT:
A process for the preparation of thrombinase. This process includes the steps of subjecting a cell free culture filtrate from a culture broth of the fermentation of Bacillus sphaericus serotype H5a5b to ultra filtration to obtain a concentrated retentate. Substantially all the protein is then salted out and the salted out protein is converted into an aqueous solution. This solution is subjected to decolorization, dialysis and lyophilization to obtain a crude powder. A solution is prepared from the crude powder and subjected to chromatography treatment to obtain a purified material which is then lyophilized.

REFERENCES:
patent: 4014860 (1977-03-01), Kakinuma et al.
patent: 4604234 (1986-08-01), Fujii et al.
patent: 5166318 (1992-11-01), Furutani et al.

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