Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-09-05
1998-07-07
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
424 931, 435 4, 435 13, 435 405, 436 86, 436501, 436811, C12N 1512
Patent
active
057767009
ABSTRACT:
An assay for determining thrombin receptor activation in a cell by measuring the internalization of a labeled antibody-thrombin receptor complex following exposure to an agonist peptide is provided. This method is useful in identifying thrombin receptor antagonists.
REFERENCES:
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Brass et al., Structure and Function of the Human Platelet Thrombin Receptor. The Journal of Biological Chemistry, vol. 267, No. 20, pp. 13795-13798, 1992.
Brass et al., Changes in the Structure and Function of the Human Thrombin Receptor during Receptor Activation, Internalization and Recycling. The Journal of Biological Chemistry, vol. 269, No. 4, pp. 2943-2952, 1994.
Woolkalis et al., Regulation of Thrombin Receptors on Human Umbilical Vein Endothelial Cells. The Journal of Biological Chemistry, vol. 270, No. 17, pp. 9868-9875, 1995.
Vu et al., Molecular Cloning of Functional Thrombin Receptor Reveals a Novel Proteolytic Mechanism of Receptor Activation. Cell, vol. 64, pp. 1057-1068, 1991.
Vassallo et al., "Structure-Function Relationships in the Activation of Platelet Thrombin Receptors by Receptor-derived Peptides", J. Biol. Chem. 1992 267, 6081-6085.
Hoxse et al "Internalization and Recycling of Activated Thrombin Receptors" 1993, vol. 268, No. 18 pp. 13756-13763 J. of Biol. Chem.
Brass Lawrence
Ramachandran Ram
Housel James C.
Nguyen Bao-Thuy L.
Trustees of the University of Pennsylvania
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