Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-12-09
1999-11-16
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436 69, 536118, G01N 3386
Patent
active
059855827
ABSTRACT:
Thrombin-based assays are disclosed for determining antithrombin III (ATIII) present in a plasma sample. The assays involve a heparin derivative which, like heparin, effectively enhances the antithrombin activity of ATIII, but which, unlike heparin, does not substantially enhance the antithrombin activity of Heparin cofactor II (HCII). In the present assay, HCII contributes about 15% or less to the determined antithrombin activity. A lyophilized reagent composition, useful in the ATIII assay and comprising thrombin and a heparin derivative, or alternatively comprising thrombin and heparin, is also disclosed. A high-calibrator plasma reference useful in the ATIII assays and in assays for other plasma constituents is also disclosed. Kits employing the several aspects of the invention are disclosed as well.
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Chin Christopher L.
Nguyen Bao-Thuy L.
Sigma-Aldrich Co.
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