Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1987-07-20
1991-07-30
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 14, 435 25, 435174, 435175, 435177, 435176, 435182, 435288, 436904, 422 56, C12Q 132, C12Q 154, C12M 140
Patent
active
050360000
ABSTRACT:
A system for quantitative colorimetric analysis of biological fluids or organic compounds, including NAD(P)H, or a substrate of an enzyme which reacts with the formation or consumption of NAD(P)H. Concentrations of organic substrates for example alcohol, cholesterol, uric acid, in a biological fluid such as saliva, blood or urine may be determined. The system gives a digital reading of the organic material the concentration which is sought to be determined; the concentration of NAD(P)H is determined by a color change or color "signal" when the NAD(P)H is above a threshold concentration and by the absence of a color signal when the concentration of NAD(P)H is below the threshold concentration. The system includes a chromogen, an electron-accepting reactant which, until exhausted, prevents a visible color change due to accumulation of reduced chromogen, and a catalyst. The system is capable of measuring colorimetrically without dilution concentrations of organic compounds in biological fluids which previously could not be measured in such concentration.
REFERENCES:
patent: 3964871 (1976-06-01), Hochstrasser
patent: 4629697 (1986-12-01), Limbach et al.
Palmer John L.
Timmerman Marsha W.
Enzymatics, Inc.
Kepplinger Esther L.
Scheiner Toni R.
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