Three-phase partitioning method for purifying a protein

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

Reexamination Certificate

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Reexamination Certificate

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07439337

ABSTRACT:
The present invention is a kit and method for purifying a protein of interest.

REFERENCES:
Sharma et al., “Purification of alkaline phospatase from chicken intestine by three-phase partitioning and use of phenyl-Sepharose 6B in the batch mode,” Bioseparation 9, 2000, pp. 155-161.
Keilin et al., “Purification of Horse-Radish Peroxidase and Comparison of its Properties with those of Catalase and Methaemoglobin”, J. Biochem. 1951 49:88-104.
Kenten et al., “A Simple Method for the Preparation of Horseradish Peroxidase”, J. Biochem. 1954 57:347-348.
Shannon et al., “Peroxidase Isozymes from Horseradish Roots”, J. Biol. Chem. 1966 241(9):2166-2171.

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