Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1994-05-17
1998-06-30
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435 14, 435 25, 435 27, 435179, 435180, 435182, 435288, 435817, 204403, C12N 1102, C12N 1104, C12Q 154, C12M 140
Patent
active
057732701
ABSTRACT:
A system for electrochemical measurement of glucose concentration in an undiluted test sample, e.g. blood is provided containing a sensor including a three-layered contiguous membrane. The membrane has a thickness of 50 to 130 microns, and is composed of a 1 to 10 micron thick first layer, a 10 to 30 micron thick second layer having an average pore diameter of 15 nanometers and a 40 to 80 micron thick third layer containing glucose oxidase. The third layer is less dense than the first and second layers and the first layer is more dense than the second layer. The layers of the membrane are fused together such that no clear distinction can be made between the layers at the boundary. The sensor is calibrated in a standard glucose solution which includes catalase as a hydrogen peroxide scavenger, and the sensor has a response that is linear throughout the concentration range of glucose in an undiluted sample. In one embodiment, the system is a polarographic cell structure containing an electrically insulating receptacle, an electrode mounted in the receptacle, and the three-layered membrane.
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D'Orazio Paul A.
Sogin David
Blackburn Robert P.
Blodgett Gerry A.
Chiron Diagnostics Corporation
Morgenstern Arthur S.
Naff David M.
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