Three-dimensional skin culture system

Details Three-dimensional skin culture system Three-dimensional skin culture system

1990-08-30

1993-11-30

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352401, 4352402, 435284, C12N 500, C12M 300

Patent

active

052664805

ABSTRACT:
The present invention relates to a three-dimensional cell culture system which can be used to culture a variety of different cells and tissues in vitro for prolonged periods of time. In accordance with the invention, cells derived from a desired tissue are inoculated and grown on a pre-established stromal support matrix. The stromal support matrix comprises stromal cells, such as fibroblasts actively growing on a three-dimensional matrix. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal matrix provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts found in vivo.

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