Three dimensional protein mapping

Data processing: measuring – calibrating – or testing – Measurement system in a specific environment – Biological or biochemical

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

Reexamination Certificate

active

06931325

ABSTRACT:
The present invention relates to multi-phase protein separation methods capable of resolving and characterizing large numbers of cellular proteins, including methods for efficiently facilitating the transfer of protein samples between separation phases. In particular, the present invention provides systems and methods for the generation of multi-dimensional protein maps. The present invention thus provides improved methods for the analysis of samples containing large numbers of proteins.

REFERENCES:
patent: 5131998 (1992-07-01), Jorgenson et al.
patent: 5498545 (1996-03-01), Vestal et al.
patent: 5670054 (1997-09-01), Kibbey et al.
patent: 6002127 (1999-12-01), Vestal et al.
patent: 6139734 (2000-10-01), Settlage et al.
patent: 6315905 (2001-11-01), Settlage et al.
patent: 2002/0039747 (2002-04-01), Lubman et al.
patent: 2002/0098595 (2002-07-01), Lubman et al.
patent: 2003/0058239 (2003-03-01), Wall et al.
patent: 2003/0143647 (2003-07-01), Finn et al.
patent: 0617 048 (1994-09-01), None
patent: WO 97/01755 (1997-01-01), None
patent: WO 98/23950 (1998-04-01), None
patent: WO 98/40395 (1998-09-01), None
patent: WO 01/58925 (2001-08-01), None
patent: WO 02088701 (2002-11-01), None
patent: WO 0158926 (2003-03-01), None
patent: WO 0159460 (2003-03-01), None
Brunger et al. Crystallography & NMR System: A new software Suite for Macromolecular Structure Determination. Acta Crystallographica. Sep. 1, 2000, vol. D54, Part 9, pp. 905-921.
U.S. Appl. No. 09/778,496, 20020039747, Lubman et al. filed Feb. 7, 2001.
U.S. Appl. No. 09/778,548, 20020098595 A1, Lubman et al. filed Feb. 7, 2001.
U.S. Appl. No. 09/968,930, Lubman et al. filed Oct. 10, 2001.
U.S. Appl. No. 10/133,711, 20030058239, Wall et al. filed Apr. 26, 2002.
U.S. Appl. No. 10/448,564, Lubman et al. filed May 30, 2003.
Aebersold et al., Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose, Proc Natl Acad Sci U S A, 84(20):6970-6974 (1987).
Anderson et al.,A two-dimensional gel database of rat liver proteins useful in gene regulation and drug effects studies, Electrophoresis, 12(11):907-930 (1991).
Andrews et al., Analysis of DNA adducts using high-performance separation techniques coupled to electrospray ionization mass spectrometry, J Chromatogr A, 856(1-2):515-526 (1999).
Ayala et al., Use of Rotofor preparative isoelectrofocusing cell in protein purification procedure, Appl Biochem Biotechnol, 69(1):11-16 (1998).
Ball et al., Purification of synthetic peptides with the aid of reversible chromatographic probes, J Chromatogr A, 686(73-83 (1994).
Bini et al., Protein expression profiles in human breast ductal carcinoma and histologically normal tissue, Electrophoresis, 18(15):2832-2841 (1997).
Binz et al., A molecular scanner to automate proteomic research and to display proteome images, Anal Chem, 71(21):4981-4988 (1999).
Brown and Cooper, Regulation, substrates and functions of src, Biochim Biophys Acta, 1287(2-3):121-149 (1996).
Chen et al., Identification of proteins from two-dimensional gel electrophoresis of human erythroleukemia cells using capillary high performance liquid chromatography/electrospray-ion trap-reflectron time-of-flight mass spectrometry with two-dimensional topographic map analysis of in-gel tryptic digest products, Rapid Commun Mass Spectrom, 13(19):1907-1916 (1999).
Chen et al., Rapid identification and screening of proteins from whole cell lysates of human erythroleukemia cells in the liquid phase, using non-porous reversed phase high-performance liquid chromatography separations of proteins followed by matrix-assisted [correction of multi-assisted] laser desorption/ionization mass spectrometry analysis and sequence database searching, Rapid Commun Mass Spectrom, 12(24):1994-2003 (1998).
Chong et al., Rapid screening of protein profiles of human breast cancer cell lines using non-porous reversed-phase high performance liquid chromatography separation with matrix-assisted laser desorption/ionization time-of-flight mass spectral analysis, Rapid Commun Mass Spectrom, 13(18):1808-1812 (1999).
Chong et al., Rapid profiling ofE. coliproteins up to 500 kDa from whole cell lysates using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Rapid Commun Mass Spectrom, 11(17):1900-1908 (1997).
Clauser et al., Role of accurate mass measurement (+/− 10 ppm) in protein identification strategies employing MS or MS/MS and database searching, Anal Chem, 71(14):2871-2882 (1999).
Cohen and Chait, Mass spectrometry of whole proteins eluted from sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels, Anal Biochem, 247(2):257-267 (1997).
Courchesne et al., Comparison of in-gel and on-membrane digestion methods at low to sub-pmol level for subsequent peptide and fragment-ion mass analysis using matrix-assisted laser-desorption/ionization mass spectrometry, Electrophoresis, 18(3-4):369-381 (1997).
Dai et al., Two-layer sample preparation: a method for MALDI-MS analysis of complex peptide and protein mixtures, Anal Chem, 71(5):1087-1091 (1999).
Damerval, Quantification of silver-strained proteins resolved by two-dimensional electrophoresis: genetic variability as related to abundance and solubility in two maize lines, Electrophoresis, 15(12):1573-1579 (1994).
Davidsson and Nilsson, Peptide mapping of proteins in cerebrospinal fluid utilizing a rapid preparative two-dimensional electrophoretic procedure and matrix-assisted laser desorption/ionization mass spectrometry, Biochim Biophys Acta, 1473(2-3):391-399 (1999).
Davidsson et al., Characterization of proteins from human cerebrospinal fluid by a combination of preparative two-dimensional liquid-phase electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Anal Chem, 71(3):642-647 (1999).
Dawson et al., MCF10AT: a model for the evolution of cancer from proliferative breast disease, Am J Pathol, 148(1):313-319 (1996.
Egan et al., Activation of Src in human breast tumor cell lines: elevated levels of phosphotyrosine phosphatase activity that preferentially recognizes the Src carboxy terminal negative regulatory tyrosine 530, Oncogene, 18(5):1227-1237 (1999).
Figeys and Aebersold, Nanoflow solvent gradient delivery from a microfabricated device for protein identifications by electrospray ionization mass spectrometry, Anal Chem, 70(18):3721-3727 (1998) (abstract only).
Figeys et al., Data-dependent modulation of solid-phase extraction capillary electrophoresis for the analysis of complex peptide and phosphopeptide mixtures by tandem mass spectrometry: application to endothelial nitric oxide synthase, Anal Chem, 71(13):2279-2287 (1999) (abstract only).
Figeys et al., Protein identification by solid phase microextraction-capillary zone electrophoresis-microelectrospray-tandem mass spectrometry, Nat Biotechnol, 14(11):1579-1583 (1996) (abstract only).
Figeys et al., An integrated microfluidics-tandem mass spectrometry system for automated protein analysis, Anal Chem, 70(18):3728-3734 (1998) (abstract only).
Figeys et al., Mirofabricated device coupled with an electrospray ionization quadrupole time-of-flight mass spectrometer: protein identifications based on enhanced-resolution mass spectrometry and tandem mass spectrometry data, Rapid Commun Mass Spectrom, 12(20):1435-1444 (1998) (abstract only).
Figeys et al., A microfabricated device for rapid protein identification by microelectrospray ion trap mass spectrometry, Anal Chem, 69(16):3153-3160 (1997) (abstract only).
Figeys et al., Optimization of solid phase microextraction—capillary zone electrophoresis—mass spectrometry for high sensitivity protein identification, Electrophoresis, 19(13):2338-2347 (1998) (abstract only).
Flyer et al., Identification by mass spectrometry of CD8(+)-T-cell Mycobacterium tuberculosis epitopes within the Rv0341 gene product, Infect Immun, 70(6):2926-2932 (2002).
Fuqua et al., Induction of the estrogen-regulated “24K” protein by heat shock, Cancer Res, 49(15):4126-4129 (1989).
Gast

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Three dimensional protein mapping does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Three dimensional protein mapping, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Three dimensional protein mapping will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3464448

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.