Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1995-06-05
1999-05-11
Stucker, Jeffrey
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 11, 424572, 424574, 623 15, C12N5/00
Patent
active
059027410
ABSTRACT:
The present invention relates to a method of stimulating the proliferation and appropriate cell maturation of a variety of different cells and tissues in three-dimensional cultures in vitro using TGF-.beta. in the culture medium. In accordance with the invention, stromal cells, including, but not limited to, chondrocytes, chondrocyte-progenitors, fibroblasts, fibroblast-like cells, umbilical cord cells or bone marrow cells from umbilical cord blood are inoculated and grown on a three-dimensional framework in the presence of TGF-.beta.. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal cells and connective tissue proteins naturally secreted by the stromal cells attach to and substantially envelope the framework composed of a biocompatible non-living material formed into a three-dimensional structure having interstitial spaces bridged by the stromal cells. The living stromal tissue so formed provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture and/or cultures implanted in vivo. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts in vivo.
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Dunkelman Noushin
Naughton Brian A.
Naughton Gail K.
Purchio Anthony F.
Zimber Michael
Advanced Tissue Sciences Inc.
Park Hankyel T.
Stucker Jeffrey
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