Three-dimensional blood-brain barrier cell and tissue culture sy

Details Three-dimensional blood-brain barrier cell and tissue culture sy Three-dimensional blood-brain barrier cell and tissue culture sy

1995-04-06

1996-11-26

Czaja, Donald E.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 11, 435 29, 435 32, 43524023, 4352401, 4352402, 4352841, 435 12, 4352891, 424572, 424422, 424484, 424 937, C12N 500, C12Q 102, A01N 102

Patent

active

055784852

ABSTRACT:
The present invention relates to a three-dimensional cell culture system which can be used to culture a variety of different cells and tissues in vitro for prolonged periods of time. In accordance with the invention, cells derived from a desired tissue are inoculated and grown on a pre-established stromal support matrix. The stromal support matrix comprises stromal cells, such as fibroblasts actively growing on a three-dimensional matrix. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal matrix provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts found in vivo.

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