Thioredoxin shufflease and use thereof

Details Thioredoxin shufflease and use thereof Thioredoxin shufflease and use thereof

1986-08-08

1990-02-27

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435189, 935 29, C12N 906, C12N 902

Patent

active

049046020

ABSTRACT:
The subject invention concerns a novel enzyme named thioredoxin shufflease, means for preparing the same, and procedures for using thioredoxin shufflease to fold proteins containing disulfide crosslinks. Thioredoxin shufflease is a generic term to define enzymes which have the following characteristics: (a) contain a single reactive thiol group; (b) catalyze the exchange of disulfides in a protein undergoing the refolding process; and (c) are not consumed in the oxidation/refolding process. Specifically exemplified is a thioredoxin shufflease produced from an E. coli thioredoxin gene.

REFERENCES:
patent: 4421685 (1983-12-01), Chance et al.
Morgenstern, R., et al, (1983) Chemical Abstracts 99:135967k.
Enzyme Nomenclature (1978) Academic Press, New York, pp. 186-189.
Holmgren, A. (1981) TIBS, 26-29.
Wallace, B. J. and Kushner, S. R. (1984) "Genetic and physical analysis of the thioredoxin (trxA) gene of Escherichia coli K-12" 32:399-408.

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