Thionin staining and imaging technique

Details Thionin staining and imaging technique Thionin staining and imaging technique

1991-11-25

1992-12-01

Housel, James C.

Chemistry: analytical and immunological testing

Biological cellular material tested

436166, 436172, 436174, 424 3, 382 6, 356 36, 356 51, G01N 130, G01N 2100

Patent

active

051680661

ABSTRACT:
Cellular analysis in accordance with the invention involves staining cellular material with a thionin stain to distinguish the nuclear portion from the cytoplasm, counterstaining, illuminating the thionin-stained cellular material with energy in the infrared spectrum, and imaging the stained cellular material. The analysis can be automated or manual.

REFERENCES:
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patent: 4595582 (1986-06-01), Balogh et al.
patent: 4998284 (1991-03-01), Bacus et al.
Oud, P. S. "The use of Light Green and Orange II as quantitative protein stains, and their combination with the Feulgen method for the simultaneous determination of protein and DNA" Histochemistry, vol. 80 (1984) pp. 49-57.
MacAuley et al., Analytical and Quantitative Cytology and Histology, vol. II, Feb. 1989, pp. 53-58.
Oud et al., Cytometry 7:8-17 (1986).
Stormer et al., Acta Histochem. 79:127-134 (1986).
Garbay et al., Analytical and Quantitative Cytology and Histology, vol. 8, Mar. 1986, pp. 25-34.
Oud et al., Analytical and Quantitative Cytology, vol. 6, No. 2, Jun. 1984, pp. 131-138.
Oud et al., Analytical and Quantitative Cytology, vol. 3, No. 4, Dec. 1981, pp. 289-294.
Holmquist et al., The Journal of Histochemistry and Cytochemistry, vol. 24, No. 12, 1976, pp. 1218-1224.
Oud, Doctoral Thesis, University of Nijmegen, Netherlands, 1986.
Zahniser, Doctoral Thesis, University of Nijmegen, Netherlands, 1979.
Puchtler, H. et al., "Infrared Fluorescence Microscopy of Stained Tissues: Principles and Technique", Histochemistry, vol. 68 (1980) pp. 211-230.

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