Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – In an organic compound
Patent
1998-06-29
2000-01-04
Dodson, Shelley A.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
In an organic compound
424 111, 424 165, 424 91, 424 149, 4241301, 5303871, A61K 5100, A61M 3614
Patent
active
060106808
ABSTRACT:
A method of radiolabeling a protein with a radionuclide including contacting the protein with a protected tertiary thiol-containing bifunctional chelating agent that is capable of reacting with the protein at one end of the agent and is capable of complexing with a radionuclide at the other end of the agent, to form a protein-acetyl-t-thiol-containing conjugate. The protein-acetyl-t-thiol-containing conjugate then is deprotected and admixed with a reducing agent for the radionuclide, where the radionuclide is added in a subsequent step, to form a mixture of reducing agent and protein-t-thiol-containing conjugate. This mixture then is reacted with a radionuclide whereby the radionuclide reacts with pendant sulfhydryl groups present on the protein-t-thiol-containing conjugate. Methods of radioimmunotherapy and diagnostic kits suitable for forming a composition to be administered to a human patient also are disclosed.
REFERENCES:
patent: 3198781 (1965-08-01), Benesch et al.
patent: 4323546 (1982-04-01), Crockford et al.
patent: 4424200 (1984-01-01), Crockford et al.
patent: 4883842 (1989-11-01), Chervu et al.
patent: 4980147 (1990-12-01), Fritzberg et al.
patent: 5061641 (1991-10-01), Shochat
patent: 5071636 (1991-12-01), Yamauchi et al.
patent: 5080884 (1992-01-01), McBride et al.
patent: 5082930 (1992-01-01), Nicolotti et al.
patent: 5102990 (1992-04-01), Rhodes
patent: 5128119 (1992-07-01), Griffiths
patent: 5162505 (1992-11-01), Dean et al.
patent: 5180816 (1993-01-01), Dean
patent: 5196515 (1993-03-01), Levar et al.
patent: 5225180 (1993-07-01), Dean et al.
patent: 5310536 (1994-05-01), Srinivasan
patent: 5426190 (1995-06-01), Govindan et al.
patent: 5443815 (1995-08-01), Dean et al.
patent: 5493031 (1996-02-01), Govindan
patent: 5496533 (1996-03-01), Jackson et al.
patent: 5508020 (1996-04-01), Dean et al.
patent: 5534497 (1996-07-01), Verbruggen et al.
patent: 5772981 (1998-06-01), Govindan et al.
Greenfield, et al., Bioconjugate Chem., 1: 400-410 (1990)"Thiol-Containing Cross-Linking Agent with Enhanced Steric Hinderance".
McGraw-Hill Dictionary of Chemical Terms, Ed. Sybil Parker, 1984.
Khaw et al--J. Nucl. Med., 23:1011-19 (1982), "Technetium-99m Labeling of Antibodies to Cardiac Myosin Fab and to Human Fibrinogen."
Khaw, et al.--Science, 209:295-97 (1980), "Myocardial infarct Imaging of Antibodies to Canine Cardiac Myosin with Indium-111-Diethylenetriamine Pentaacetic Acid."
Krejcarek, et al.--Biochem. Byophys. Res. Commun., 77:581-85 (1977), "Covalent Attachment of Chelating Groups to Macromolecules."
Childs, et al.--J. Nucl. Med., 26:293 (1985), "Optimum Conditons for Labeling of DTPA Coupled Antibodies with Technetium-99m."
Fritzberg et al.--J. Nucl. Med., 27:957 (1986), "Radiopharmaceutical Chemistry v. Antibodies."
Baidoo et al. Cancer Research (Supp.) 50:799s-803s(1990), "Tc Labeling of Protein: Initial Evaluation of a Novel Diaminedithiol Bifunctional Chelating Agent."
Wong-Chemistry of Protein Conjugation and Cross-Linking, CRC Press, Inc., Boca Raton, Florida, pp. 17-23 (1991), "Reactive Groups of Proteins and Their Modifying Agents."
McCall et al.--Bioconjugate Chem. 1:222-26 (1990), "Simplified Method for Conjugating Macrocyclic Bifunctional Chelating Agents to Antibodies via 2-Iminothiolane."
Goff et al. Bioconjugate Chem. 1:381-86(1990), "Substituted 2-Iminothiolanes: Reagents for the Preparation of Disulfide Cross-Linked Congjugagtes with Increased Stability."
Govindan Seregulam V.
Grebenau Ruth
Griffiths Gary L.
Hansen Hans J.
Dodson Shelley A.
Immunomedics Inc.
Jones Dameron
LandOfFree
Thiolation of proteins for radionuclide-based radioimmunodetecti does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Thiolation of proteins for radionuclide-based radioimmunodetecti, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Thiolation of proteins for radionuclide-based radioimmunodetecti will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1070308