Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1994-03-23
1995-02-07
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435264, C12N 948, D06M 1600
Patent
active
053875176
ABSTRACT:
A purified thiol activated protease obtained from bromelain stem extract is provided. The protease has a molecular weight of about 17,000 daltons to about 21,000 daltons and an isoelectric point of about pI 4.5 to about pI 5.0. Methods for obtaining this enzyme and methods for use of this enzyme for debridement of eschar tissue are disclosed.
REFERENCES:
patent: 2977287 (1961-03-01), Bloch et al.
patent: 3940478 (1976-02-01), Kurtz et al.
patent: 3983209 (1976-09-01), Schmitt
patent: 4122158 (1978-10-01), Schmitt
patent: 4197291 (1980-04-01), Klein et al.
patent: 4226854 (1980-10-01), Klein et al.
patent: 4276281 (1981-06-01), Crikelair
patent: 4286064 (1981-08-01), Galbraith
patent: 4307081 (1981-12-01), Klein et al.
patent: 4329430 (1982-05-01), Klein et al.
patent: 4361551 (1982-11-01), Galbraith
patent: 4613502 (1986-09-01), Turkova et al.
patent: 4645668 (1987-02-01), Pinnell
patent: 4732758 (1988-03-01), Hurion et al.
patent: 4801451 (1989-01-01), Hellgren et al.
patent: 5106621 (1992-04-01), Rowan et al.
Feinstein et al., (1964) Biochemistry, 3(8), 1050-1054, "On the Molecular Weights of the Proteolytic Enzymes of Stem Bromelain".
Ota et al., (1985) J. Biochem., 98(1), 219-228, "Reinvestigation of Fractionation and Some Properties of the Proteotytically Active Components of Stem and Fruit Bromelains".
Vesterberg, O. et al., Isoelectric Fractionation, Analysis and Characterization of Ampholytes in Natural pH Gradients; Acta Chem. Scandinavica 20 (1966) pp. 820-834.
Rowan, A. D. et al., Ananain: A Novel Cysteine Proteinase Found in Pineapple Stem; Archives of Biochemistry and Biophysics., vol. 257, Nov. 15, 1988, pp. 262-270.
Ethicon Inc.
Robinson Douglas W.
Weber Jon P.
Woodrow Hal Brent
LandOfFree
Thiol activated protease from stem bromelain for treating devita does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Thiol activated protease from stem bromelain for treating devita, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Thiol activated protease from stem bromelain for treating devita will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1109848