Thiohydantoin formation and selective modification of the carbox

Details Thiohydantoin formation and selective modification of the carbox Thiohydantoin formation and selective modification of the carbox

1994-07-08

1997-09-09

Lukton, David

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

436 89, 530340, 530345, A61K 3802

Patent

active

056656033

ABSTRACT:
A method of forming a thiohydantoin from an N-protected amino acid is described. The method employs a phosphate compound selected from the group consisting of

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patent: 5304497 (1994-04-01), Boyd et al.
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Meuth et al., "Stepwise sequence determination from thecarboxyl terminus of peptides," Biochemistry, 21: 3750-3757 (1982).
Kenner et al., "Selective removal of the C-terminal residue as a thiohydantoin. The use of diphenyl phosphorisocyanatidate," Peptides. Part IV. pp. 673-678 (1953).
Boyd et al., "Sequencing of peptides and proteins from the carboxy terminus," Anal. Biochemistry, 206: 344-352 (1992).

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