Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2005-03-15
2005-03-15
Zitomer, Stephanie (Department: 1634)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C530S350000
Reexamination Certificate
active
06867289
ABSTRACT:
This invention generally relates the generation of aptamers and to the use of aptamers as diagnostic and therapeutic agents. More particularly, the present invention relates to methods using combinatorial chemistry to prepare aptamers having controlled thiophosphate replacement in the phosphate backbone for improved binding efficiencies to a target and to RNA and/or DNA products having novel nucleotide sequences and enhanced target binding efficacies.
REFERENCES:
patent: 5218088 (1993-06-01), Gorenstein et al.
patent: 5397698 (1995-03-01), Goodman et al.
patent: 5576302 (1996-11-01), Cook et al.
patent: 5587361 (1996-12-01), Cook et al.
patent: 5599797 (1997-02-01), Cook et al.
patent: 5602000 (1997-02-01), Hyman
patent: 5607923 (1997-03-01), Cook et al.
patent: 5620963 (1997-04-01), Cook et al.
patent: 5635488 (1997-06-01), Cook et al.
patent: 5639873 (1997-06-01), Barascut et al.
patent: 5660985 (1997-08-01), Kirschenheuter et al.
patent: 5661134 (1997-08-01), Cook et al.
patent: 5705337 (1998-01-01), Gold et al.
patent: 5734041 (1998-03-01), Just et al.
patent: 5763595 (1998-06-01), Gold et al.
patent: 5795721 (1998-08-01), Rabin et al.
patent: 5804445 (1998-09-01), Brasier
patent: 5874219 (1999-02-01), Rava et al.
patent: WO 92 14842 (1992-09-01), None
patent: WO 9214843 (1992-09-01), None
patent: WO 93 08296 (1993-04-01), None
patent: WO 96 19572 (1996-06-01), None
Selection and Characterization of an RNA Decoy for Transcription Factor NF-xB,Biochemistry1999, 38, pp. 3168-3174.
Novel Combinatorial Selection of Phosphorothioate Oligonucleotide Aptamers,Biochemistry1998, 37, pp. 16489-16493.
Oligonucleotide Analogues as Potential Chemotherapeutic Agents,Pharmaceutical Research, vol. 5, No. 9, 1988, pp. 539-549.
Bielinska A et al: “Regulation of Gene Expression with Double-Stranded Phosphorothioate Oligonucleotides” Science, American Association for the Advancement of Science,, US, vol. 250, Nov. 16, 1990, pp. 997-1000, XP002065516 ISSN: 0036-8075.
Kunsch, Charles et al: “Selection of optimal.kappa.B/Rel DNA-binding motifs: interaction of bot subunits of NF-.kappa.B with DNA is required for transcriptional activation” Molecular and Cellular Biology ( 1992 ), 12(10), 4412-2, XP009003474.
Antsypovich, Sergei et al: “Cross-linked DNA duplexes. Exonuclease stability and interactio with the nucleic transcription factor of the .kappa. light-chai enhancer (NF-. kappa. B )” European Journal of Biochemistry (1998), 255(2), 414-421, XP002226148.
Sharma, H.W. et al.: “Transcription factor decoy approach to decipher the role of NF-KB in oncogenesis” Anticancer Research, vol. 16, 1996, pp. 61-70, XP009003192.
Morishita, R.M. et al.: “In vivo transfection of cis element “decoy” against nuclear factor-kB binding site prevents myocardial infarction” Nature Medicine, vol. 3, No. 8, 1997, pp. 894-899, XP009003193.
Khaled, Annette R. et al: “Use of phosphorothioate -modified oligodeoxynucleotides to inhibit NF-kappa. B expression and lymphocyte function” Clinical Immunology and Immunopathology (1998), 86(2), 170-179, XP009003515.
Stec, W.J. et al.: “Deoxyribonucleoside 3′-0-(2-thio-and 2-oxo-“spiro”-4,4-pentamethylene-1,3,2-oxathiaphospholane)s” J. Am. Chem. Soc. , vol. 120, 1998, pp. 7156-7167, XP002239290.
Uhlmann E et al: “Studies on the Mechanism of Stabilization of Partially Phosphorothioated Oligonucleotides Against Nucleolytic Degradation” Antisense & Nucleic Acid Drug Development, Mary Ann Liebert, Inc., New York, US, vol. 7, No. 4, Aug. 1997, pp. 345-350, XP001117604 ISSN: 1087-2906.
Nakamaye, Kay L. et al: “Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside.alpha.-thiotriphosphates” Nucleic Acids Research (1988), 16(21), 9947-59, 1988, XP001097564.
Aronson Judy
Gorenstein David G.
Herzog Norbert
Luxon Bruce A.
Board of Regents The University of Texas Systems
Chalker Flores LLP
Flores Edwin S.
Zitomer Stephanie
LandOfFree
Thio-modified aptamer synthetic methods and compositions does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Thio-modified aptamer synthetic methods and compositions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Thio-modified aptamer synthetic methods and compositions will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3409367