Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1991-11-18
1994-07-12
Warden, Robert J.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
435287, 435288, 435817, 422 681, 422 69, 20415312, C12M 134, C12M 140, G01N 3096, C25F 700
Patent
active
053288475
ABSTRACT:
A modular biosensor system for chemical or biological agent detection utilizes electrochemical measurement of an ion current across a gate membrane triggered by the reaction of the target agent with a recognition protein conjugated to a channel blocker. The sensor system includes a bioresponse simulator or "biochemical switch" module which contains the recognition protein-channel blocker conjugate, and in which the detection reactions occur, and a transducer module which contains a gate membrane and a measuring electrode, and in which the presence of agent is sensed electrically. In the poised state, ion channels in the gate membrane are blocked by the recognition protein-channel blocker conjugate. Detection reactions remove the recognition protein-channel blocker conjugate from the ion channels, thus eliciting an ion current surge in the gate membrane which subsequently triggers an output alarm. Sufficiently large currents are generated that simple direct current electronics are adequate for the measurements. The biosensor has applications for environmental, medical, and industrial use.
REFERENCES:
patent: 3966580 (1976-06-01), Janata et al.
patent: 4214968 (1980-07-01), Battaglia
patent: 4343782 (1982-08-01), Shapiro
patent: 4634599 (1987-01-01), Uzgiris
patent: 4637861 (1987-01-01), Krull et al.
patent: 4661422 (1987-04-01), Marianowski
patent: 4661442 (1987-04-01), Lukens
patent: 4776944 (1988-10-01), Janata et al.
Lin, et al,. "Characterization of Immobilized Antibodies on Silica Surfaces," IEEE Transaction on Biomedical Engineering, vol. 35 (No. 6).
Krull, et al, "Langmuir-Blodgett Technology and Receptor Action in Stabilized Lipid Membranes," in Fundamentals and Applications of Chem.
Janata, et al, "Chemical Sensors," Analytical Chemistry, vol. 60, pp. 62R-74R, 1988 Zin particular pp. 67R-68R).
Wohtjen, "Chemical Microsensors and Microinstrumentation," Analytical Chemistry, vol. 55: pp. 98A-108A, 1984.
Krull, "Ion-Current Signal Optimization for Lipid Membrane Based Biosensors," Analytical Chimica Acta, vol. 192: pp. 321-326, 1987.
Fritsche, "Clemiluminescence Method for the Determination of Nanogram Amounts of Highly Toxic Alkylphosphates," Analytica Chimical Acta.
Case, "Magnetic Resonance Studies of the Mitochondrial Divalent Cation Carrier," Biochiemica et Biophysica Actas, vol. 375: pp. 69-86.
Caras, et al, "Enzymatically Sensitive Field Effect Transistors," Methods in Enzymology, vol. 137: pp. 247.gtoreq.255, 1988.
Neher, et al, "Single-channel currents recorded from membrane of denervated ffog muscle fibers," Nature, vol. 260: pp. 799-802, 1976.
DeFelice, Introduction to Membrane Noise, pp. 386-407, Plenum Press, 1981.
Feher, et al, "Fluctuation Spectroscopy: Determination of Chemical Reaction Kinetics from the Frequency Spectrum of Fluctuations," Proc. N. Stryer, Biochemistry (3rd Ed.), pp. 288-293 and 964-969 Freeman.
Urban, et al, "Ion Movements in Gramicidin Pores: An example of Single-File Transport," (Biochimica et Biophysica Acta), vol. 602: pp. 331-354.
Shi, et al, "Action of calcium channel and beta-adrenergic blocking agents in bilayer lipid membranes," Biochimica et Biophysica Acta, vol. Clicheportiche et al., "Synthesis of new, highly radioactive tetrodotoxin derivatives and their binding properties to the sodium channel,".
Krueger, et al, "Block of sodium channels in planar lipid bilayers by guanidium toxins and calcium": Are the mechanisms of voltage dependence.
Montal et al., "Channel properties of the purified acetylcholine receptor from T. Californica reconstituted in planar lipid bilayer membranes".
Angelides et al., "Spatial relations of the neurotoxin binding sites on the sodium channel," Ann. N.Y. Acad. Sci. 221 (1987).
Hub et al., "Polymerization of lipid and lysolapid like diacetylenes in monolayers and liosomes," J. Macromol. Sci.-Chem. A15(5):701-715 (1981).
Krull et al., "Langmuir-Blodgett film characteristics and phospholipid membrane ion conduction," Analytica Chimica Acta 174:83-94 (1985).
Case George D.
Worley, III Jennings F.
Kim Christopher Y.
Warden Robert J.
LandOfFree
Thin membrane sensor with biochemical switch does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Thin membrane sensor with biochemical switch, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Thin membrane sensor with biochemical switch will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-395955