Thin film membrane enzyme/coemzyme reactor and method of using s

Details Thin film membrane enzyme/coemzyme reactor and method of using s Thin film membrane enzyme/coemzyme reactor and method of using s

1989-04-07

1991-10-15

Lacey, David L.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435180, 435176, 435177, 435288, 435817, 210632, C12N 1102, C12N 1108, C12N 1114

Patent

active

050574213

ABSTRACT:
An enzyme reactor system is provided based on the entrapment of a coenzyme-requiring enzyme, a coenzyme, and a regeneration enzyme in a hydrogel layer coated on a support, and confined by an ultraporous thin film semipermeable membrane. The diffusion barrier confines the coenzyme-requiring enzyme, coenzyme, and regeneration enzyme but lets substrate and reaction products, exclusive of coenzyme, diffuse freely into and out of the hydrogel layer. In an alternate embodiment, the support is formed of an ultraporous thin film semipermeable membrane on a microporous or macroporous support, through which the reaction products, exclusive of coenzyme, can diffuse freely, but through which neither coenzyme-requiring enzyme, coenzyme, regeneration enzyme, nor substrate can pass. In this embodiment, the product is recovered in high purity, free of substrate, coenzyme-requiring enzyme, coenzyme, and regeneration enzyme. A constant supply of coenzyme for the enzyme reaction is assured by initially charging the hydrogel layer with an adequate supply of coenzyme. The problem of coenzyme depletion is solved by including a second enzyme in the hydrogel layer which regenerates the used coenzyme, thus recycling it. The substrates for both the primary and regeneration reactions are supplied in the circulating solvent. Reaction products, exclusive of coenzyme, are carried away by the solvent.

REFERENCES:
patent: 4956289 (1990-09-01), Wrasidlo et al.

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