Thick-film conductivity enzyme electrodes in a vertical stack as

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

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204403, 435174, 435176, 435177, 435180, 435817, C12M 140, G01N 27327, G01N 1100, G01N 1114

Patent

active

057704392

ABSTRACT:
A biosensor is produced by depositing thick film pastes by screen-printing on a substrate to produce thick-film conductivity electrodes, and immobilizing enzymes on the electrodes. In specific embodiments, the substrate is an unfired ceramic, a fired ceramic or a plastic, the paste is a noble metal paste, enzyme immobilization is with a bifunctional reagent, a plurality of different enzymes are immobilized on different electrodes, and/or the electrodes are printed as parallel lines or in the image of a comb or concentric circles. A multi-dimensional biosensor is prepared by forming thick film paste electrodes containing an immobilized enzyme on a plurality of separate substrates, disposing the separate substrates one on top of the other in a vertical stack, pressing the stacked substrates together and curing the paste.

REFERENCES:
patent: 4721601 (1988-01-01), Wrigton et al.
patent: 5063081 (1991-11-01), Cozzette et al.
Takatsu, et. al., Sensor and Activators, 11 (1987) pp. 309-317.

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