Thermostable xanthine oxidase from Arthrobacter luteus

Details Thermostable xanthine oxidase from Arthrobacter luteus Thermostable xanthine oxidase from Arthrobacter luteus

1991-04-16

1993-02-09

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435830, C12N 906, C12N 100

Patent

active

051852578

ABSTRACT:
A new thermostable xanthine oxidase obtained from a microbe belonging to the genus Arthrobacter and a method of its production are disclosed. The present invention affords a xanthine oxidase which is excellent in thermal stability and which retains at least 70% residual activity after heat treatment at 60.degree. C. for 30 minutes. Arthrobacter luteus ATCC 21606 is the preferred microbe. The enzyme has a molecular weight of about 160,000 daltons as determined by gel filtration and a pH optimum of about 7.5.

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Woolfolk et al, J. of Bacteriology, vol. 135, No. 2, pp. 422-428, 1978.
Woolfolk et al, J. of Bacteriology, vol. 130, No. 3, pp. 1175-1191, 1977.
Machida et al, Agric. Biol. Chem., vol. 45, No. 2, pp. 425-432, 1981.

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