Thermostable ribonuclease H obtained from Archeoglobus...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S320100, C435S252300, C435S325000, C435S069100, C435S019000, C530S350000, C536S023200

Reexamination Certificate

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07572617

ABSTRACT:
An isolated polypeptide having a thermostable ribonuclease H activity fromArchaeoglobus profundusis highly useful in genetic engineering, as well as a gene encoding this polypeptide and a genetic engineering process for producing the polypeptide.

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Chapados, et al, “Structural Biochemistry of a Type 2 RNase H: RNA primer recognition and removal during DNA replication”, J. Mol. Biol., 307:541-556. (2001).
Itaya, Mitsuhiro and Kanae Kondo, “Molecular cloning of a ribonuclease H (RNase HI) gene from an extreme thermophileThermus thermophilusHB8: a thermostable RNase H can functionally replace theEscherichia colienzyme in vivo”, Nucleic Acids Research, 19(16):4443-4449. (1991).
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Lopez-Garcia, et al, “Plasmid pGS5 from the Hyperthermophilic AchaeonArchaeoglobus profundusis negatively supercoiled”, Journal of Bacteriology, 182(17):4998-5000. (Sep. 2000).

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