Thermostable proteolytic enzymes and uses thereof in peptide and

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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435212, 435219, 435220, 536 232, C12P 2106

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active

061435171

ABSTRACT:
The subject invention pertains to new thermostable enzymes and the use of these enzymes both in proteolysis as well as protein and polypeptide synthesis. The subject invention further concerns polynucleotide sequences which encode the enzymes of the subject invention.

REFERENCES:
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Eggen, Rik, Geerling, Ans, Watts, Jennifer and de Vos, Willem M., "Characterization of Pyrolysin, a Hyperthermoactive Serine Protease from the Archaebacterium Pyrococcus furiosus" FEMS Microbiology Letters, 71:17-20 (1990).
Voorhorst, Wilfried G.B., Eggen, Rik I.L., Geerling, Ans C.M., Platteeuw, Christ, Siezen, Roland J., de Vos, Willem M., "Isolation and Characterization of the Hyperthermostable Serine Protease, Pyrolysin, and its Gene from the Hyperthermophilic Archaeon Pyrococcus furiosus." Journal of Biological Chemistry, 271,34:20426-20431 (1996).
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Halio, Sheryl B., Blumentals, Stephen A. Short, Barbara M. Merrill, Robert M. Kelly (1996) "Sequence, Expression in Escherichia coli, and analysis of the Gene Encoding a Novel Intracellullar Protease (Pfpl) from the Hyperthermophilic Archaeon Pyrococcus furiosus" Journal of Bacteriology 178(9)::2605-2612.
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