Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-04-07
1996-12-24
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 435 5, 53038826, 530350, C12Q 168, C12Q 170, C12P 1934, C07K 1640
Patent
active
055872875
ABSTRACT:
Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature T.sub.1 which is generally below about 85.degree. C. The inhibitors are irreversibly inactivated at temperature T.sub.2 which is generally above about 40.degree. C. T.sub.2 is also greater than T.sub.1. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR.
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Stratagene Catalog (1988) p. 39.
Harlow, E & D. Lane, Antibodies in Laboratory Manual (1988) pp. 27, 141, 142, 231, 236, 237.
Bobrovnik, S. A. et al. Dynamics and Mechanism of Antibody Thermoinactivation Biopolim. Kletka (1988) 4:310-313.
Christy, Jr. Kenneth G.
Daiss John L.
Esders Theodore W.
Scalice Edward R.
Sharkey David J.
Johnson & Johnson Clinical Diagnostics Inc.
Rees Dianne
Zitomer Stephanie W.
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