Thermostable polymerase

Details Thermostable polymerase Thermostable polymerase

1998-06-11

2000-10-10

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 435183, C12Q 168

Patent

active

061300454

ABSTRACT:
A thermostable enzyme having polymerase activity and substantially no nuclease activity is provided. The thermostable enzyme is characterized by comprising a sequence of nine amino acid residues at least proximal to the N-terminus that has less than 50% but greater than 40% amino acid sequence identity with residues 280 to 288 of the naturally occurring Thermus aquaticus polymerase. The subject enzyme finds use in a variety of different application in which polynucleotides are enzymatically produced, particularly in PCR based applications.

REFERENCES:
patent: H1531 (1996-05-01), Blumentals et al.
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5079352 (1992-01-01), Gelfand et al.
patent: 5108892 (1992-04-01), Burke et al.
patent: 5352600 (1994-10-01), Gelfand et al.
patent: 5405774 (1995-04-01), Abramsom et al.
patent: 5436149 (1995-07-01), Barnes
patent: 5455170 (1995-10-01), Abramsom et al.
patent: 5466591 (1995-11-01), Abramsom et al.
patent: 5556772 (1996-09-01), Sorge et al.
patent: 5616494 (1997-04-01), Barnes
patent: 5674738 (1997-10-01), Abramsom et al.

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