Thermostable Photinus pyralis luciferase mutant

Details Thermostable Photinus pyralis luciferase mutant Thermostable Photinus pyralis luciferase mutant

2011-03-15

2011-03-15

Steadman, David J (Department: 1656)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

C435S189000, C435S320100, C435S252300, C435S419000, C536S023200, C800S295000

Reexamination Certificate

active

07906298

ABSTRACT:
A protein having luciferase activity and at least 60% similarity to luciferase fromPhotinus pyralis, Luciola mingrelica, Luciola cruciataorLuciola lateralis. Hotaria paroula, Pyrophorus plagiophtalamus, Lampyris noctiluca, Pyrocoelia nayakoorPhotinus pennsylanvanicawherein in the sequence of the enzyme, at least one of (a) the amino acid residue corresponding to residue 214 inPhotinus pyralisluciferase; (b) the amino acid residue corresponding to residue 232 inPhotinus pyralisluciferase; (c) the amino acid residue corresponding to residue 295 inPhotinus pyralisluciferase; (d) the amino acid residue corresponding to acid 14 ofPhotinus pyralisluciferase; (e) the amino acid residue corresponding to amino acid 35 ofPhotinus pyralisluciferase; (f) the amino acid residue corresponding to amino acid residue 105 ofPhotinus pyralisluciferase; (g) the amino acid residue corresponding to amino acid residue 234 ofPhotinus pyralisluciferase; (h) the amino acid residue corresponding to amino acid residue 420 ofPhotinus pyralisluciferase; (i) the amino acid residue corresponding to amino acid residue 310 ofPhotinus pyralisluciferase; is different to the amino acid which appears in the corresponding wild type sequence and wherein the luciferase enzyme has increased thermostability as compared to an enzyme having the amino acid of the corresponding wild-type luciferase at this position.

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