Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2004-08-11
2008-10-14
Pak, Yong D (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S440000, C435S025000, C435S069100, C435S071100, C536S023200
Reexamination Certificate
active
07435570
ABSTRACT:
The invention relates to novel variants of cytochrome P450 oxygenases. These variants have at least one mutation improving their ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. The variants also have at least one mutation improving thermostability as compared to the parent enzyme or corresponding wild-type enzyme. Preferred variants include cytochrome P450 BM-3 heme domain variants having L52I, I58V, F87A, H100R, S106R, F107L, A135S, M145A/V, A184V, N239H, S274T, L324I, V340M, I366V, K434E, E442K, and/or V446I amino acid substitutions.
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Arnold Frances H.
Cirino Patrick C.
Baker, Jr. Joseph R.
California Institute of Technology
Gavrilovich Dodd & Lindsey LLP
Pak Yong D
LandOfFree
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