Thermostable nucleic acid polymerase from Thermococcus...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

Reexamination Certificate

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C435S194000, C435S183000, C435S091100, C435S091200, C530S350000, C536S023100, C536S023200

Reexamination Certificate

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07425423

ABSTRACT:
A purified thermostable enzyme is derived from the thermophilic archaebacteriumThermococcus gorgonarius.The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5322785 (1994-06-01), Comb et al.
patent: 5352778 (1994-10-01), Comb et al.
patent: 5436149 (1995-07-01), Barnes
patent: 0 455 430 (1991-11-01), None
patent: 0 201 184 (1992-12-01), None
patent: 0 200 362 (1993-01-01), None
patent: 0 547 920 (1993-06-01), None
patent: 0 546 920 (1997-03-01), None
patent: 0 258 017 (1997-06-01), None
patent: 0 693 078 (1999-06-01), None
patent: WO 94/26766 (1994-11-01), None
Balch et al., “Methanogens: Reevaluation of a Unique Biological Group”,Microbiological Reviews, 1979, 43: 260-296.
Bernad et al., “A Conserved 3′-5′ Exonuclease Active Site in Prokaryotic and Eukaryotic DNA Polymerases”,Cell, 1989, 59: 219-228.
Bessman et al., “Enzymatic Synthesis of Deoxyribonucleic Acid”,Journal of Biological Chemistry, 1957, 233: 171-177.
Braithwaite and Ito, “Compilation, alignment, and phylogenetic relationships of DNA Polymerases”,Nucleic Acids Research, 1993, 21: 787-802.
Brinkmann et al., “High-level expression of recombinant genes inEscherichia coliis dependent on the availability of thednaYgene product”,Gene, 1989, 85: 109-114.
Buttin and Kornberg, “Enzymatic Synthesis of Deoxyribonucleic Acid”,Journal of Biological Chemistry, 1966, 241: 5419-5427.
Cariello et al., “Fidelity ofThermococcus litoralisDNA polymerase (Vent™) in PCR determined by denaturing gradient gel electrophoresis”,Nucleic Acids Research, 1991, 19: 4193-4198.
Chien et al, “Deoxyribonucleic Acid Polymerase from the Extreme ThermophileThermus aquaticus”, Journal of Bacteriology, 1976, 127: 1550-1557.
Flaman et al., “A rapid PCR fidelity assay”,Nucleic Acids Research, 1994, 22: 3259-3260.
Frey and Suppmann, “Demonstration of the Expand™ PCR System's Greater Fidelity and Higher Yields with alacI-based PCR Fidelity Assay”,Biochemica, 1995, 2: 8-9.
Frey and Suppmann, “Demonstration of the Expand™ PCR System's Greater Fidelity and Higher Yields with alacI-based PCR Fidelity Assay”,Biochemica Information, Nr. 96, 1995, pp. 21-23.
Höltke et al., “Sensitive Chemiluminescent Detection of Digoxigenin-Labeled Nucleic Acids: A Fast and Simple Protocol and Its Applications”,Biotechniques, 1992, 12: 104-113.
Keohavong and Thilly, “Fidelity of DNA polymerases in DNA amplification”,Proceedings of National Academy of Science USA, 1989, 86: 9253-9257.
Lawyer et al., “Isolation, Characterization and Expression inEscherichia coliof the DNA Polymerase Gene fromThermus aquaticus” The Journal of Biological Chemistry, 1989, 264: 6427-6437.
Lehman et al., “Enzymatic Synthesis of Deoxyribonucleic Acid”,The Journal of Biological Chemistry, 1958, 233: 163-170.
Ling et al., “Optimization of the Polymerase Chain Reaction with Regard to Fidelity: Modified T7,Taq, and Vent DNA Polymerases”PCR Methods and Applications, 1991, 1: 63-69.
Lundberg et al., “High-fidelity amplification using a thermostable DNA polymerase isolated fromPyrococcis furiosus”, Gene, 1991, 108: 1-6.
Mattila et al., “Fidelity of DNA synthesis by theThermococcus litoralisDNA polymerase—an extremely heat stable enzyme with proofreading activity”,Nucleic Acids Research, 1991, 19: 4967-4973.
Ochman et al., “Amplification of Flanking Sequences by Inverse PCR”,PCR Protocols: A Guide to Methods and Applications, 1990, pp. 219-227.
Provost et al., “Transgenic systems for in vivo mutation analysis”,Mutation Research, 1993, 288: 133-149.
Raleigh et al., “McrA and McrB restriction phenotypes of someE. colistrains and implifications for gene cloning”,Nucleic Acids Research, 1988, 16: 1563-1575.
Rosenberg et al., “Vectors for selective expression of cloned DNAs by T7 RNA polymerase”,Gene, 1987, 56: 125-135.
Spanos and Hübscher, “Recovery of Functional Proteins in Sodium Dodecyl Sulfate Gels”,Methods in Enzymology, 1983, 91: 263-277.
Studier et al., “Use of T7 RNA Polymerase to Direct Expression of Cloned Genes”,Methods of Enzymology, 1990, 185: 60-89.

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