Thermostable nucleic acid polymerase from Thermococcus...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

Reexamination Certificate

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C435S194000, C435S183000, C435S091100, C435S091200, C530S350000, C536S023100, C536S023200

Reexamination Certificate

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09269860

ABSTRACT:
A purified thermostable enzyme is derived from the thermophilic archaebacteriumThermococcus gorgonarius.The enzyme can be native or recombinant, retains approximately 90% of its activity after incubation for two hours at 95° C. in the presence of stabilizing agents and possesses 3′-5′ proofreading exonuclease activity. Thermostable DNA polymerases are useful in many recombinant DNA techniques, especially nucleic acid amplification by the polymerase chain reaction (PCR).

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