Thermostable luciferases and methods of production

Details Thermostable luciferases and methods of production Thermostable luciferases and methods of production

2009-07-31

2011-10-04

Prouty, Rebecca (Department: 1652)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

C435S174000, C435S189000, C536S023200

Reexamination Certificate

active

08030017

ABSTRACT:
Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.

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