Thermostable duplex-specific nuclease

Details Thermostable duplex-specific nuclease Thermostable duplex-specific nuclease

2011-05-31

2011-05-31

Fronda, Christian L (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S183000, C435S252300, C435S320100, C536S023200

Reexamination Certificate

active

07951569

ABSTRACT:
The present invention provides a thermostable duplex-specific nuclease (DSN) and a method for digesting a nucleic acid using said DSN, namely, a protein having a Brachyura-derived duplex-specific nuclease activity, a gene encoding for said protein, a recombinant vector comprising said gene and a transformed cell or transduced cell comprising said vector and a method for producing a protein having a duplex-specific nuclease activity, which comprises culturing said transformed cell or transduced cell using a medium and collecting the protein having a duplex-specific nuclease activity from the cultured mixture, a method for digesting a nucleic acid using said protein having duplex-specific nuclease activity, a method for detecting RNA using said DSN and a reagent kit to be used in the aforementioned methods.

REFERENCES:
patent: 2005/0164216 (2005-07-01), Lukyanov et al.
Chica et al. Curr Opin Biotechnol. Aug. 2005;16(4):378-84.
Sen et al. Appl Biochem Biotechnol. Dec. 2007;143(3):212-23.
Veronika E. Anisimova, et al. “Is crab duplex-specific nuclease a member of theSerratiafamily of non-specific nucleases?”, GENE, vol. 418, No. 1-2, XP22716638A, Jul. 15, 2008, pp. 41-48.
Veronika E. Anisimova, et al., “Isolation, characterization and molecular cloning of Duplex-Specific Nuclease from the hepatopancreas of the Kamchatka crab”, BMC Biochemistry, vol. 9, No. 14, XP002555506, May 21, 2008, pp. 1-12.
Dmitry A. Shagin, et al., “A Novel Method for SNP Detection Using a New Duplex-Specific Nuclease From Crab Hepatopancreas”, Genome Research, vol. 12, No. 12, XP002555507, Dec. 2002, pp. 1935-1942.
Pavel A. Zhulidov, et al., “Simple cDNA normalization using kamchatka crab duplex-specific nuclease”, Nucleic Acids Research, vol. 32, No. 3, XP002555508, Feb. 18, 2004, pp. 1-8.
V.E. Anisimova, et al., “Renaturation, Activation, and Practical Use of Recombinant Duplex-Specific Nuclease from Kamchatka Crab”, Biochemistry (Moscow), vol. 71, No. 5, XP002555510, May 2006, pp. 513-519.
Veronika E. Anisimova, et al., “Thermolabile duplex-specific nuclease” Biotechnology Letters, vol. 31, No. 2, XP002555509, Sep. 23, 2008. pp. 251-257.
Office Action issued Oct. 25, 2010, in European Patent Application No. 09 169 259.0.

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