Thermostable DNA Topoisomerase V

Details Thermostable DNA Topoisomerase V Thermostable DNA Topoisomerase V

1993-03-24

1995-06-27

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435233, 536 221, C12P 1934, C12N 990, C07H 2104

Patent

active

054279283

ABSTRACT:
A type 1 topoisomerase, designated topoisomerase V, has been isolated and substantially purified from the halophilic thermophilic methanogen bacterium Methanopyrus kandleri. The topoisomerase was purified by a process including the steps of lysing cells of M. kandleri to form a lysate, treating the lysate with polyethyleneimine to form a precipitate and a supernatant, precipitating the polyethyleneimine supernatant with ammonium sulfate, chromatographing the ammonium sulfate precipitate on phosphocellulose to produce a phosphocellulose eluate, chromatographing the phosphocellulose eluate on heparin to produce a heparin eluate, and chromatographing the heparin eluate on a column capable of separating proteins by molecular size therein to produce a substantially purified thermostable DNA topoisomerase V. Topoisomerase V can relax DNA and can unlink DNA by reducing the linking number of closed circular DNA. Topoisomerase V is active over a wide range of temperatures and ionic conditions and does not require magnesium or ATP for its activity. It is recognized by anti-human topoisomerase I antibody.

REFERENCES:
patent: 4894456 (1990-01-01), Wall et al.
patent: 4963658 (1990-10-01), Kung et al.
patent: 5070192 (1991-12-01), Earnshaw et al.
patent: 5106742 (1992-04-01), Wall et al.
patent: 5122526 (1992-06-01), Wall et al.
patent: 5244903 (1993-09-01), Wall et al.
Slesarev et al. (1991) J. Biol. Chem., 266(19), 12321-12328.
Kovalsky et al. (1990) Nucl. Acid. Res., 18(9), 2801-2805.
de la Tour et al. (1991) J. Bacteriol., 173(12), 3921-3923.
Huber et al. (1989) Nature, 342, 833-834.
Slesarev et al. (1993) Nature, 364, 735-737.
Cowan (1992) TIBTECH, 10, 315-323.
Andera et al. (1993) FEMS Microbiol. Lett., 110(1), 107-112.
Kurr et al. (1991) Arch. Microbiol., 156(4), 239-247.
Bashkin et al. (1993) Biochemistry, 32, 1895-1898.
Vemura et al. (1987) Nucl. Acid. Res., 15(23), 9727-9739.
Eng et al. (1989) J. Biol. Chem., 264(23), 13373-13376.
Kelly et al. (1988) Biotechnol. Prog., 4(2), 47-62.
Borman (Nov. 4, 1991) Chem. Eng. News., 31-34.
Burggraf et al. (1991) Syst. Appl. Microbiol., 14(4), 346-351 in Biol. Abst., 93(7), Ref. No. 72587.
J. C. Wang & L. F. Liu, "DNA Replication: Topological Aspects and the Roles of DNA Topoisomerases," in DNA Topology and Its Biological Effects (N. R. Cozzarelli & J. C. Wang, eds., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1990), ch. 11, pp. 321-340.
W. M. Huang, "Virus-Encoded DNA Topoisomerases," in DNA Topology and Its Biological Effects (N. R. Cozzarelli & J. C. Wang, eds., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. 1990), ch. 8, pp. 265-284.
M. D. Frank-Kamenetskii, "DNA Supercoiling and Unusual Structures," in DNA Topology and Its Biological Effects (N. R. Cozzarelli & J. C. Wang, eds., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1990), ch. 5, pp. 185-215.
E. C. Lau et al., "Preparation of Denatured Plasmid Templates for PCR Amplication," Biotechniques 14:378 (1993).
J. Kato et al., "Purification and Characterization of DNA Topoisomerase IV in Escherichia Coli," J. Biol. Chem. 267:25676-25684 (1992).
A. Kikuchi et al., "Reverse Gyrase and DNA Supercoiling in Sulfolobus," System Appl. Microbiol. 7:72-78 (1986).
B. S. Glisson & W. E. Ross, "DNA Topoisomerase II: A Primer on the Enzyme and Its Unique Role as a Multidrug Target in Cancer Chemotherapy," Pharmac. Ther. 32:89-106 (1987).
P. O. Brown & N. R. Cozzarelli, "A Sign Inversion Mechanism for Enzymatic Supercoiling of DNA," Science 206:1081-1083 (1979).
P. Benedetti et al., "The Use of Yeast and Yeast Strains Expressing Human DNA Topoisomerases in the Study of Anticancer Drugs," in Drug Resistance as a Biochemical Target in Cancer Chemotherapy (T. Tsuruo & M. Ogawa, eds., Academic Press, San Diego, 1992) ch. 7, pp. 129-145.
P. D'Arpa et al., "Mechanisms of Tumor Cell Killing by Topoisomerase Poisons," in Drug Resistance as a Biochemical Target in Cancer Chemotherapy (T. Tsuruo & M. Ogawa, eds., Academic Press, San Diego, 1992) ch. 8, pp. 147-163.
A. L. Bodley & L. F. Liu, "Topoisomerases as Novel Targets for Cancer Chemotherapy," Bio/Technology 6:1315-1319 (1988).
C. Boutier de la Tour et al., "Reverse Gyrase in Thermophilic Eubacteria," J. Bact. 173:3921-3923 (1991).
L. F. Liu, "DNA Topoisomerase Poisons as Antitumor Drugs," Annu. Rev. Biochem. 58:351-375 (1989).
S. M. Adams & R. Blakesley, "Linear Amplication DNA Sequencing," Focus 13:56-58 (1991).
J. C. Wang, "DNA Topoisomerases," Annu. Rev. Biochem. 54:665-697 (1985).
M. Gellert, "DNA Topoisomerases," Annu. Rev. Biochem. 50:879-910 (1981).
Y. Zivanovic et al., "Chromatin Reconstitution on Small DNA Rings III. Histone H5 Dependence of DNA Supercoiling in the Nucleosome," J. Mol. Biol. 214:479-495 (1990).
P. D'Arpa et al., "Use of Molecular Cloning Methods to Map the Distribution of Epitopes on Topoisomerase I (Scl-70) Recognized by Sera of Scleroderma Patients," Arthritis Rheum. 33:1501-1511 (1990).
P. F. Agris et al., "Plant DNA Topoisomerase I Is Recognized and Inhibited by Human Scl-70 Sera Autoantibodies," Exp. Cell Res. 189:276-279 (1990).
P. Bernard & M. Couturier, "Cell Killing by the F Plasmid CcdB Protein Involves Poisoning of DNA-Topoisomerase II Complexes," J. Mol. Biol. 226:735-745 (1992).
H. Tamura et al., "Molecular Cloning of a cDNA of a Camptothecin-Resistant Human DNA Topoisomerase I and Identification of Mutation Sites," Nucl. Acids Res. 19:69-75 (1991).
N. Osheroff, "Biochemical Basis for the Interactions of Type I and Type II Topoisomerases with DNA," Pharmac. Ther. 41:223-241 (1989).
P. A. Der Garabedian et al., "Mg.sup.2+, Asp. and Glu Effects in the Processive and Distribution DNA Relaxation Catalyzed by a Eukaryotic Topoisomerase I," Biochemistry 30:9940-9947 (1991).
J. Bashkin et al., "Structure of DNA in a Nucleosome Core at High Salt Concentration and at High Temperature," Biochemistry 32:1895-1898 (1993).
R. K. Saiki et al., "Enzymatic Amplication of .beta.-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sicle Cell Anemia," Science 230:1350-1354 (1985).
P. D'Arpa et al., "cDNA Cloning of Human DNA Topoisomerase I: Catalytic Activity of a 67.7 kDa Carboxyl-Terminal Fragment," Proc. Natl. Acad. Sci. USA 85:2543-2547 (1988).
M. D. Been & J. J. Champoux, "DNA Breakage and Closure by Rat Liver Type I Topoisomerase: Separation of the Half-Reactions by Using a Single-Stranded DNA Substrate," Proc. Natl. Acad. Sci. USA 78:2883-2887 (1981).
B. F. Pugh et al., "Extent of Duplex DNA Underwinding Induced by RecA Protein Binding in the Presence of ATP," J. Mol. Biol. 205:487-492 (1989).
C. A. Prada & K. J. Marians, "Transcriptional Activation of pBR322 DNA Can Lead to Duplex DNA Unwinding Catalyzed by the Escherichia coli Preprimosome," J. Biol. Chem. 264:15120-15129 (1989).
B. F. Pugh & M. M. Cox, "recA Protein Binding to the Heteroduplex Product of DNA Strand Exchange," J. Biol. Chem. 262:1337-1343 (1987).
T. A. Baker et al., "Extensive Unwinding of the Plasmid Template During Staged Enzymatic Initiation of DNA Replication from the Origin of the Escherichia coli Chromosome," Cell 45:53-64 (1986).
A. M. Wu et al., "Unwinding Associated with Synapses of DNA Molecules by recA Protein," Proc. Natl. Acad. Sci. USA 80:1256-1260 (1983).
U. H. Stettler et al., "Preparation and Characterization of Form V DNA, the Duplex DNA Resulting From Association of Complementary, Circular Single-Stranded DNA," J. Mol. Biol. 131:21-40 (1979).
M. Iwabuchi et al., "ATP-Dependent Unwinding of the Double Helix and Extensive Supercoiling by Escherichia coli recA Protein in the Presence of Topoisomerase," J. Biol. Chem. 258:12394-12404 (1983).
F. Barany, "Genetic Disease Detection and DNA Amplication Using Cloned Thermostable Ligase," Proc. Natl. Acad. Sci. USA 88:189-193 (1991).
D. R. Barker, "A More Robust, Rapid Alkaline Denaturation Sequencing Method," Biotechniques 14:168-169 (1993).
K. Hsiao, "A Fast and Simple Procedure for Sequencing Double Stranded DNA with Sequence," Nucl. Acids Res. 19:2787 (1991).
G. Pan et al., "Ligation of Synthetic Activated DNA Substrates by S

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