Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2003-01-30
2009-10-06
Sitton, Jehanne S (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
07598032
ABSTRACT:
Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are advantageous in many in vitro DNA synthesis applications. Such enzymes are particularly useful for use in chain termination nucleic acid sequencing protocols, as are native forms of such enzymes. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.
REFERENCES:
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5338678 (1994-08-01), Senter et al.
patent: 5418155 (1995-05-01), Cormier et al.
patent: 5466591 (1995-11-01), Abramson et al.
patent: 5545548 (1996-08-01), Senter et al.
patent: 5571706 (1996-11-01), Baker et al.
patent: 5614365 (1997-03-01), Tabor et al.
patent: 5723298 (1998-03-01), Oommen et al.
patent: 5852191 (1998-12-01), Karandikar et al.
patent: 5939292 (1999-08-01), Gelfand et al.
patent: 6083686 (2000-07-01), Sullivan
patent: 6107029 (2000-08-01), Giordano
patent: 6265193 (2001-07-01), Brandis et al.
patent: 2002/0164591 (2002-11-01), Brandis et al.
patent: 2006/0088879 (2006-04-01), Brandis et al.
patent: 0 402 108 (1990-12-01), None
patent: 0 482 714 (1992-02-01), None
patent: 0 655 506 (1994-11-01), None
patent: 0727496 (1996-08-01), None
patent: 0 823 479 (1997-07-01), None
patent: 0727496 (2000-09-01), None
patent: WO 98/40496 (1998-03-01), None
Kalman, L.V. Genome Sci. Technol. 1:42, 1995.
Lomize (Lomize et al, Proteins: Structure, Function and Genetics, suppl. 3, pp. 199-203, 1999 : A Prediction of Protein Structure: The Problem of Fold Multiplicity).
Beese et al., “Crystal structures of the Klenow Fragment of DNA polymerase I complexed with Deoxynucleoside Triphosphate and Pyrophosphate,”Biochemistry, 1993, pp. 14095-14101, vol. 32.
Delarue et al., “An attempt to unify the structure of polymerases,”Protein Engineering, 1990.
Donlin et al., “Mutants affecting nycleotide recognition by T7 DNA polymerase,”Biochemistry, 1994, pp. 14908-14917, vol. 33.
Ito, et al., Compilation and alignment of DNA polymerase sequences,Nucleic Acids Research, 1991, pp. 4045-4057, vol. 19.
Lee, et al., “DNA sequencing with dye labeled terminators and t7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments,”Nucleic Acids Research, 1992, pp. 2471-2483, vol. 20.
Lomize, et al., “Prediction of protein structure: the problem of fold multiplicity,”Proteins: Structure, Function and Genetics, 1999, pp. 199-203, Suppl. 3.
Polesky et al., “Side chains involved in catalysis of the polymerase reaction of DNA polymerase I fromEscherichia coli,” The Journal of Biological Chemistry, 1992, pp. 8417-8428, vol. 267.
Reeve, et al., “A Novel Thermostable Polymerase for DNA Sequencing,”Nature, 1995, pp. 796-797, vol. 376.
Sanger, et al., “DNA Sequencing with chain-terminating inhibition,”PNAS, 1977, pp. 5483-5467, vol. 74.
Voss, et al., “Automated Cycle Sequencing with Taquenase: Protocols for Internal Labeling, Dye Primer and “Doublex” Simultaneous Sequencing,”Biotechniques, 1997, pp. 312-318, vol. 23.
Gelfand David Harrow
Kalman Lisa Vivian
Myers Thomas W.
Reichert Fred Lawrence
Sigua Christopher Lim
Roche Molecular Systems
Sitton Jehanne S
Townsend and Townsend / and Crew LLP
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