Thermostable DNA polymerases having reduced discrimination again

Details Thermostable DNA polymerases having reduced discrimination again Thermostable DNA polymerases having reduced discrimination again

1997-08-05

1999-08-17

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435194, 536 232, C12P 1934, C12N 912, C07H 2104

Patent

active

059392924

ABSTRACT:
Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides, such as ribonucleotides, into DNA products, are advantageous in many in vitro synthesis applications. Such enzymes are particularly useful for use in nucleic acid sequencing protocols and provide novel means for DNA sequence analysis. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.

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