Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-08-05
1999-08-17
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435194, 536 232, C12P 1934, C12N 912, C07H 2104
Patent
active
059392924
ABSTRACT:
Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides, such as ribonucleotides, into DNA products, are advantageous in many in vitro synthesis applications. Such enzymes are particularly useful for use in nucleic acid sequencing protocols and provide novel means for DNA sequence analysis. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.
REFERENCES:
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5466591 (1995-11-01), Abramson et al.
patent: 5614365 (1997-03-01), Tabor et al.
Berg et al., 1963, Informational Macromolecular, NY Academic Press:467-483.
Van De Sande et al., 1972, J. Biol. Chem. 247 (19):6140-6148.
Sanger et al., 1977, Proc. Natl. Acad. Sci. 74:5463.
Barnes, 1978, J. Mol. Biol. 119:83-99.
Ludwig, 1986, Biophosphates and Their Analogues, Elsevier.
Parvin et al., 1986, DNA 5 (2):167-171.
Nakamaye, 1988, Nucleic Acids Research 16 (21):9947-9959.
Murray, 1989, Nucleic Acids Research 17 (21):8889.
Lee et al., 1992, Nucleic Acids Research 20 (10):2471-2483.
Polesky, 1992, J. Biol. Chem., 267 (12):8417-8428.
Akhmetzjanov and Vakhitov, 1992, Nucleic Acids Research 20 (21): 5839.
Walder et al., 1993, Nucleic Acids Research 21 (18):4339-4343.
Donlin and Johnson, 1994, Biochemistry 33:14908-14917.
Sousa and Padilla, 1995, EMBO J. 14 (18):4609-4621.
Delarue et al. "An Attempt to Unify the Structure of Polymerases", Prot. Eng. 3(6):461-467, 1990.
Gelfand David Harrow
Kalman Lisa Vivian
Reichert Fred Lawrence
Petry Douglas A.
Roche Molecular Systems Inc.
Stole Einar
Wax Robert A.
LandOfFree
Thermostable DNA polymerases having reduced discrimination again does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Thermostable DNA polymerases having reduced discrimination again, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Thermostable DNA polymerases having reduced discrimination again will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-314406