Thermostable DNA polymerases

Details Thermostable DNA polymerases Thermostable DNA polymerases

1996-05-14

1999-03-23

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

435194, 435 911, 435 912, C12N 900, C12N 912, C12N 1934

Patent

active

058858130

ABSTRACT:
An enzymatically active DNA polymerase having between 540 and 582 amino acids having a tyrosine at a position equivalent to position 667 of Taq DNA polymerase, wherein said polymerase lacks 5' to 3' exonuclease activity, and wherein said polymerase has at least 95% homology in its amino acid sequence to the DNA polymerase of Thermus aquaticus, Thermus flavus or Thermus thermophilus, and wherein said polymerase forms a single polypeptide band on an SDS PAGE.

REFERENCES:
patent: 4795699 (1989-01-01), Tabor et al.
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 5075216 (1991-12-01), Innis et al.
patent: 5079352 (1992-01-01), Gelfand et al.
patent: 5436149 (1995-07-01), Barnes
patent: 5466591 (1995-11-01), Abramson et al.
patent: 5474920 (1995-12-01), Moses
Barnes, "The fidelity of Taq polymerase catalizing PCR is improved by an N-terminal deletion," Gene 112;29-35 (1992).
Reeve and Fuller, "A novel thermostable polymerase for DNA sequencing," Nature 376:796-797 (1995).
Samols et al., "Thermo Sequenase; a new thermostable DNA polymerase for DNA sequencing," Amersham Life Science, Editorial Comments 22:29-36 (1995).
Tabor and Richardson, "A single residue in DNA polymerases of the Escherichia coli DNA polymerase l family is critical for distinguishing between deoxy and dideoxyribonucleotides," Proc. Natl. Acad. Sci. USA 92:6339-6343 (1995).
Phang et al. (1995) Gene 163:65-68.
Gutman et al. (1993) J. Bacteriol. 175:3581-3590.
Asakura et al. (1993) J. Frement. Bioengin. 76:265-269.
Lawyer et al. (1989) J. Biol. Chem. 264:6427-6437.
Akhmetzjanov et al. (1992) Nucl. Acids Res. 20:5839.
Kim et al. (1995) Nature 376:612-616.

Affiliated with

Also associated with

Rating

Thermostable DNA polymerases does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Thermostable DNA polymerases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Thermostable DNA polymerases will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2124184