Thermostable DNA polymerase from Thermoanaerobacter thermohydros

Details Thermostable DNA polymerase from Thermoanaerobacter thermohydros Thermostable DNA polymerase from Thermoanaerobacter thermohydros

1996-12-13

1998-04-28

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 4352523, 435194, 435325, 435419, 536 232, C12N 912, C12N 1554, C12P 1934, C12Q 168

Patent

active

057443122

ABSTRACT:
An enzymatically active DNA polymerase or fragment thereof having at least 80% homology in its amino acid sequence to at least a contiguous 40 amino acid sequence of the DNA polymerase of Thermoanaerobacter thermohydrosulfuricus.

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patent: 5173411 (1992-12-01), Tabor et al.
Joyce and Steitz, "Function and Structure Relationships in DNA Polymerases," Annu. Rev. Biochem, 63:777-822 (1994).
Kong et al., "Characterization of a DNA Polymerase from the Hyperthermophile Archaea Thermococcus litoralis, " J. Biol. Chem. 268:1965-1975 (1993).
Reddy et al., "Hyperexpression and purification of Escherichia coli adenylate cyclase using a vector designed for expression of lethal gene products," Nucleic Acids Research 17:10473-10488 (1989).
Richardson, "DNA polymerase from Escherichia coli ," Procedures in Nucleic Acid Research, Cantoni and Davies editors, Harper and Row, New York, pp. 263-276 (1966).
Scopes, Protein Purification, Springer-Verlag, New York, New York, pp. 46-48 (1994).
Uemori et al., "Cloning of the DNA Polymerase Gene of Bacillus caldotenax and Characterization of the Gene Product," J. Biochem. 113:401-410 (1993).
Walker et al., "Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system," Proc. Natl. Acad. Sci. USA 89:392-396 (1992).

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