Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1996-09-23
1998-04-07
Hendricks, Keith D.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
536 232, 536 234, 4353201, 435325, 435419, 4352523, 43525233, C12N 912, C12N 1554, C12N 1562
Patent
active
057363730
ABSTRACT:
A novel Type I DNA polymerase derived from Bacillus pallidus is provided. The gene encoding the polymerase has been cloned and expressed. Characterization of the enzyme revealed, in addition to DNA polymerase activity, 5'-3' exonuclease activity, reverse transcriptase activity, strand displacing activity and the ability to initiate polymerization at a nick in double-stranded DNA. In certain constructs the domain encoding the 5'-3' exonuclease activity is deleted to provide an enzyme which is particularly useful for Strand Displacement Amplification.
REFERENCES:
White et al., Antonie van Leeuwenhoek, vol. 64 (3/4), pp. 357-386, 1994, abstract only.
Hensel et al., Syst. Appl. Microb., 11(3):312-319, 1989, abstract only.
Scholz et al., Syst. Appl. Microbiol., 9(2-3):91-96, 1987, abstract only.
P. D. Gutman and K. W. Minton "Conserved sites in the 5'-3' exonuclease domain of Escherichia coli DNA polymerase" Nucl. Acids Res. 21:4406-4407 (1993).
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L. Blanco, et al. "A general structure for DNA-dependent DNA polymerases" Gene 100:27-38 (1991).
M. G. Riggs, et al. "Construction of single amino acid substitution mutants ofo cloned Bacillus stearothermophilus DNA polymerase I which lack 5'-3' exonuclease activity" Biochim. Biophys. Acta 1307:178-186 (1996).
A. Diaz, et al. "Streptococcus pneumoniae DNA Polymerase I Lacks 3'-5' Exonuclease Activity: Localization of the 5'-3' Exonucleolytic Domain" J. Bacteriol. 174:2014-2024 (1992, Mar.).
F. C. Lawyer, et al. "High-level Expression, PUrification, and Enzymatic Characterization of Full-length Thermus aquaticus DNA Polymerase and a Truncated Form Deficient in 5'-3' Exonuclease Activity" PCR Mtds. Appl. 2:275-287 (1993).
A. M. Phang, et al. "Cloning and complete sequence of the DNA polymerase-encoding gene (Bstpoll) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus" Gene 163:65-68 (1995).
T. Uemori, et al. "Cloning of the DNA Polymerase Gene of Bacillus caldotenax Characterization of the Gene Product" J. Biochem. 113:401-410 (1993).
Becton Dickinson and Company
Fugit Donna R.
Hendricks Keith D.
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