Thermostable diaphorase gene

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

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4352523, 43525233, 43525411, 4353201, 536 232, C12N 902, C12N 120, C12N 114, C12N 1500, C07H 2104

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061468622

ABSTRACT:
The present invention provides a gene derived from a thermophilic Bacillus, comprising the nucleotide sequence of SEQ ID No.2 and encoding a thermostable diaphorase comprising the amino acid sequence of SEQ ID No.1, a recombinant vector possessing the gene, a transformant with the recombinant vector and a process for producing the thermostable diaphorase by using the transformant.

REFERENCES:
Rudinger (1976) Characteristics of the amino acids as components of a peptide hormones sequence. In: Peptide Hormone. Ed. J. A. Parsons. University Park Press, Baltimore, MD. pp. 1-7, Jan. 1976.
Ngo et al. (1994) Computational complexity, protein structure prediction, and the ILevinthal paradox. In: The Protein Folding Problem and Tertiary Structure Prediction. Eds. Merz et al. Birkhauser et al. Boston, MA. pp. 491-495, Jan. 1994.
Thornton et al. (1995) Protein Engineering: Editorial Overview. Current Opinion in Biotechnology 6(4): 367-369, Sep. 1995.
Wallace (1993) Understanding cytochrome c function: engineering protein structure by semisynthesis. The FASEB Journal 7: 505-515., Apr. 1993.
Mains et al. (1980) Purification of an NADH-(dichlorophenol-indophenol) oxidoreductase from Bacillus stearothermophilus, Biochemical Journal. 191 (2): 457-465, Nov. 1980.
Matsudira (1991) Limited N-terminal sequence analysis. Methods in Ehzymology.182: 602-613, Feb. 1991.
Wozney (1991) Using purified protein to clone its gene. Methods in Enzymology. 182: 738-751, Feb. 1991.
Maniatis et al. (1982) Chapter 12: Vectors that express cloned DNA in Escherichia coli. In Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. pp. 404-433, Jan. 1982.
Pharmacia Catalog (1997) Catalog No. 27-4935-01. p. 87, Jan. 1997.

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