Thermostable cellulase from a thermomonospora gene

Details Thermostable cellulase from a thermomonospora gene Thermostable cellulase from a thermomonospora gene

1994-06-24

1997-10-14

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 96, 435201, 4352523, 4353201, 424 9461, 536 237, C12P 1920, C12N 926, A61K 3847, C07H 2104

Patent

active

056771517

ABSTRACT:
The invention relates to a gene isolated from Thermomonospora fusca, wherein the gene encodes a thermostable cellulase. Disclosed is the nucleotide sequence of the T. fusca gene; and nucleic acid molecules comprising the gene, or a fragment of the gene, that can be used to recombinantly express the cellulase or a catalytically active polypeptide thereof, respectively. The isolated and purified recombinant cellulase or catalytically active polypeptide may be used to hydrolyze substrate either by itself; or in combination with other cellulases, with the resultant combination having unexpected hydrolytic activity.

REFERENCES:
patent: 5232851 (1993-08-01), Cox et al.
patent: 5318905 (1994-06-01), Saito et al.
Bothwell, M.K. et al. (1993) "Synergism between pure Thermomonospora fusca and Trichoderma reesei cellulases" Biomass and Bioenergy. 4(4):293-299 1993.
Irwin et al., "Activity Studies of Eight Purified Cellulases: Specificity, Synergism, and Binding Domain Effects", Biotechnology and Bioengineering, vol. 42, 1993, pp. 1002-1013.
Walker et al., "Engineering Cellulase Mixtures by Varying the Mole Fraction of Thermomonospora fusca E.sub.5 and E.sub.3, Trichoderma reesei CBHI, and Caldocellum saccharolyticum, .beta.-Glucosidase Biotechnology and Bioengineering", vol. 42, 1993, pp. 1019-1028.
Wilson, D.B. (1992) "Biochemistry and genetics of actinomycete cellulases" Critical Rev. in Biotechnol. 12(1/2):45-63.
Woodward, J. et al. (1988) "Hydrolysis of cellulose by saturating and non-saturating concentrations of cellulase: implications for synergism" Bio/technology. 6:301-304. Mar. 1988.
Walker, L.P. et al. (1992) "Fragmentation of cellulose by the major Thermomonospora fusca cellulases, Trichoderma reesei CBHI, and their mixtures", Biotech. and Bioeng. 40:1019-1026. 1992.
Lao, G. and Wilson, D.B. (1992) "Cloning and sequencing of exocellulase and a protease from T. fusca," Abstr. Gen. Meet. Am. Soc. Microbiol. 92(0) :314.

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