Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-04-06
1996-02-13
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435832, 435278, 4352525, C12N 924, C12N 120, C12N 100, D21C 300
Patent
active
054910870
ABSTRACT:
An isolated arabinofuranosidase from Bacillus stearothermophilus NRRL B-18659, Bacillus stearothermophilus NRRL B-18660 and Bacillus stearothermophilus NRRL B-18661 is disclosed. The arabinofuranosidase has a maximum activity at about pH 6.0 and at about 65.degree. C., maintains at least about 50% of its maximum activity at 70.degree. C. and pH 7.0 after 80 minutes, and has an isoelectric point of about 4.4. The arabinfuranosidase can be used in a method of hydrolyzing xylan present in wood pulp at temperatures of at least about 60.degree. C. and a pH of at least about 7.0. The arabinofuranosidase is used along with at least two xylanases and a xylosidase isolated from the above Bacillus stearothermophilus strains.
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Elm Dana D.
Zamost Bruce L.
Agris Cheryl H.
Meller Michael V.
Naff David M.
Novo Nordisk A S
Zelson Steve T.
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