Thermostable amylases and pullulanases from two anaerobic microo

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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435201, 435210, 4352521, 435801, 435822, C12N 928

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049295578

ABSTRACT:
At least one type of excretable, thermostable amylolytic enzyme, such as amylase and pullulanase, can be recovered from a culture medium in which thermophilic anaerobic bacteria, such as Thermoanaerobacter finii or Thermobacteroides acetoethylicus, are propagated anaerobically in continuous culture, in the presence of a higher saccharide as carbon source, such an enzyme having a high stability at temperatures between 60.degree. and 70.degree. C. in absence of metal ions and substrate and under aerobic conditions.

REFERENCES:
patent: 4572898 (1986-02-01), Zeikus
patent: 4628028 (1986-12-01), Katkocin et al.
Plant et al., "Starch Degradation by Thermophilic Anaerobic Bacteria", Syst. Appl. Microbiol., vol. 9, Nos. 1-2, pp. 158-162 (1987), (CA 107: 20540g).
Koch et al., (Appl. Microbiol. Biotechnol., 27:192-198, 1987).
Hyun et al., Applied & Environmental Microbiology, 49:1168-1173, "General Biochemical Characterization of Thermostable . . . " (1985).

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