Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1990-11-21
1993-02-23
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 95, 435201, 435202, 435204, 435205, 435832, C12N 924, C12N 926, C12N 928, C12N 934
Patent
active
051889560
ABSTRACT:
A thermostable amylase having the following properties: (1) Action: acting on raw starch to produce mainly maltose and matotriose; (2) Optimum pH: about 6.0; (3) Stable pH: after incubation at a pH of 5 to 8 for one hour at 22.degree. C., the residual activity thereof is at least 95%; (4) Optimum temperature: 70.degree. C.; (5) Thermostability: the enzyme is not substantially inactivated by incubation at 60.degree. C. or 70.degree. C. for 15 minutes; and after incubation at 70.degree. C. for one hour, the residual activity thereof is at least 90%; (6) Molecular weight: 52,000.+-.5,000 as determined by gel filtration; a process for production of the amylase using Bacillus stearothermophilus; and a process for production of maltooligosaccharide comprising mainly maltose and maltotriose, comprising the steps of treating raw starch with a thermostable amylase which acts on raw starch, at a temperature lower than a temperature at which the raw starch is gelatinized to hydrolyze the starch and thereby form maltooligosaccharides comprising mainly maltose and maltotriose; and recovering the maltooligosaccharides.
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Partial European Search Report.
Nanmori Takashi
Shinke Ryu
Lilling Herbert J.
Showa Denka K.K.
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