Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Patent
1998-08-21
2000-11-21
Smith, Lynette R. F.
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
435 691, 4353201, 435415, 435419, 536 237, 800284, 800288, 800298, 800312, C12N 504, C12N 1531, C12N 1556, C12N 1582, A01H 510
Patent
active
061501719
ABSTRACT:
An isolated nucleic acid from the bacterium Thermotoga neapolitana, encoding a thermostable .alpha.-galactosidase is provided. The Thermotoga gene is cloned into a high expression vector to provide large quantities of a purified thermostable .alpha.-galactosidase. The thermostable enzyme is used in high-temperature processing of soy products to remove .alpha.-galactosides.
REFERENCES:
patent: 5543576 (1996-08-01), Van Ooijen et al.
McCabe et al, Bio/technology, vol. 6, pp. 923-926, 1988.
Michael R. King, et al. Thermostable .alpha.-galactosidase from Thermotoga neapolitana: cloning, sequencing and expression. FEMS Microbiology Letters. 1998 163: 37-42.
Guy D. Duffaud, et al. Purification and Characterization of Extremely Thermostable .beta.-Mannanase, .beta.-Mannosidase, and .alpha.-Galactosidase from the Hyperthermophilic Eubacterium Thermotoga neapolitana 5068. Applied and Environmental Microbiology. 1997 64: 169-177.
Richard Schuler, et al. Kinetic Properties of .alpha.-D-galactosidase from Lactobacillus fermenti. Enzyme Microbiology Technology. 1985 7: 207-211.
K. Schmid, et al. Raffinose Metabilism in Escherichia coli K12 Purification and Properties of a New .alpha.-Galactosidase Specified by a Transmissible Plasmid. The Journal of Biochemistry. 1976 67: 95-104.
Richard I. Somiari, et al. Properties of an extracellular glycosidase of Aspergillus niger suitable for removal of oligosacccharides from cowpea meal. Enzyme and Microbiology Technology. 1995 17: 311-316.
Roger A. Korus, et al. The use of .alpha.-Galactosidase and invertase in Hollow Fiber Reactors. Biotechnology and Bioengineering. 1977 19: 1-8.
Darunee Thananunkul, et al. Degradation of Raffinose and Stachyose in Soybean Milk by A-Galactosidase from Mortierella vinacea. Entrapment of .alpha.-galactosidase within polyacrylamide gel. The Journal of Food Science. 1976 41: 173-174.
G. Talbot, et al. Purification and Characterization of thermostable .beta.-Mannanase and .alpha.-Galactosidase from Bacillus stearothermophilus. Applied and Environmental Microbiology. 1990: 3305-3510.
Masaru Mitsutomi, et al. Isolation and identification of oligosaccharides Produced from Raffinose by Transgalactosylation Reaction of Thermostable .alpha.-Galactosidase from Pycnoporus cinnabarinus. Agriculture Biological Chemistry. 1998 52: 2305-2311.
Marisa S. Garro, et al. Purification of .alpha.-galactosidase from Lactobacillus fermentum. Journal of Biotechnology. 1996 45: 103-109.
Hiroyuki Hashimoto, et al. Purification and Some Properties of .alpha.-Galactosidase from Pseudomonas fluorescens H-601. Agriculture Biological Chemistry. 1991 55: 2831-2838.
C. Leuschner, et al. Heat-stable enzymes from extremely thermophilic and hyperthermophilic microorganisms. World Journal of Microbiology and Biotechnology. 1995 II: 95-114.
Satoshi Ishii, et al. The functional role of glutamine-280 and threonine-282 in human .alpha.-galactosidase. Biochimica et Biophysica Acta. 1995 1270: 163-167.
Alex Zhu, et al. Identification of tyrosine 108 in coffee bean .alpha.-galactosidase as an essential residue for the enzyme activity. Biochimica et Biophysica Acta. 1995 1247: 260-264.
Santiago Rios, Purification and Molecular Properties of an .alpha.-galactosidase synthesized and secreted by Aspergillus nidulans. FEMS Microbiology Letters. 1993 112: 35-42.
Susanne Zeilinger, et al. Conditions of Formation, Purification, and Characterization of an .alpha.-Galactosidase of Trichoderma reesei RUT C30. Applied and Environmental Microbiology. 1993 59: 1347-1353.
Carol M. McCutchen, et al. Characterization of Extremely Thermostable enzymatic Breakers (.alpha.-1.6-Galactosidase and .beta.-1,4-Mannanase) from the Hyperthermophilic Bacterium Thermotoga Neapolitana 5068 for Hydrolysis of Guar Gum. Biotechnology and Bioengineering. 1996 52: 332-339.
Ramachandran Murali, et al. Crystallization and Preliminary X-ray Analysis of Human .alpha.-Galactosidase A Complex. The Journal Molecular Biology. 1994 239: 578-580.
Jill E. Porter, et al. Ion Exchange and Affinity chromatography in the Scaleup of the Purification of .alpha.-Galactosidase from Soybean Seeds. Biotechnology and Bioengineering. 1991 37: 356-363.
John H. Reynolds, An Immobilized .alpha.-Galactosidase Continuous Flow Reactor Biotechnology and Bioengineering. 1974 16: 135-147.
Yoshinori Koyama, et al. Cloning of .alpha.-Galactosidase Genes from an Extreme Thermophile, Thermus Strain T2, and Their Expression in Thermus thermophilus HB27. Applied and Environmental Microbiology. 1990 56: 2251-2254.
Charalampos Aslanidis, et al. Nucleotide Sequences and Operon Structure of Plasmid-Borne Genes Mediating Uptake and Utilization of Raffinose in Escherichia ColiA. The Journal of Bacteriology. 1989 171: 6753-6763.
Kenji Sakai, et al. Hydrolysis of .alpha.-Galactosyl Oligosaccharides in Soymilk by .alpha.-Galactosidase of Bifidobacterium breve 203. Agriculture Biology Chemistry. 1987 51: 315-322.
Marisa S. Garro, et al. Characterization of Alpha-Galactosidase from Lactobacillus fermentum. The Journal of Bacteriology. 1993 75: 485-488.
Frank Gherardini, et al. Purification and Characterization of Two .alpha.-Galactosidases Associated with Catabolism of Guar and Other .alpha.-Galactosides by Bacteroides ovatus. The Journal of Bacteriology. 1985 161: 500-506.
V.H. Mulimani, et al. Enzymic Hydrolysis of Raffinose and Stachyose in Soymilk by Alpha-Galactosidase from Gibberella Fujikuroi. Biochemistry and Molecular Biology International. 1995 36: 897-905.
M.C.M. Hensing, et al. Effects of Cultivation Conditions on the Production of Heterologous .alpha.-Galactosidase by Kluyveromyces lactis. Applied Microbiology Biotechnology. 1995 43: 58-64.
K. L. Smiley, et al. Alpha-Galactosidase Production and Use in a Hollow-Fiber Reactor. Applied and Environmental Microbiology. 1976 31: 615-617.
Chassy Bruce
Eveleigh Douglas E.
King Michael R.
Yernool Dinesh
Mehta Ashwin D.
Reed Janet E.
Rutgers The State University of New Jersey
Smith Lynette R. F.
The Board of Trustees of the University of Illinois
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