Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-05-30
1997-05-27
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 243, C12Q 168, C07H 2104
Patent
active
056331380
ABSTRACT:
Alkaline phosphatase from Thermus thermophilus has been isolated. The enzyme has a pH optimum of greater than 10.5 and is stable to heating at 65.degree. C for 1 hour. The claimed invention relates to a method of detecting nucleic acids in a sample by providing a nucleic acid probe labelled with thermostable alkaline phosphatase from Thermus thermophilus; contacting the sample with said labelled nucleic acid; and detecting said nucleic acid in said sample by means of said thermostable alkaline phosphatase.
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Davis Maria
Szasz Joseph
Amersham Life Science, Inc.
Atzel Amy
Jones W. Gary
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